Literature summary for 3.1.21.7 extracted from

Dalhus, B.; Arvai, A.S.; Rosnes, I.; Olsen, O.E.; Backe, P.H.; Alseth, I.; Gao, H.; Cao, W.; Tainer, J.A.; Bjoras, M.
Structures of endonuclease V with DNA reveal initiation of deaminated adenine repair (2009), Nat. Struct. Mol. Biol., 16, 138-143.

Search for more literature for 3.1.21.7

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain BL21 CodonPlus (DE3) RIL	Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment)	Organism
EndoV in complex with a hypoxanthine lesion substrate and with product DNA, hanging drop vapour diffusion method, in 6-12% (w/v) MPEG 2k in 200 mM imidazole-matate buffer, pH 5.8-7.4	Thermotoga maritima

Protein Variants

Protein Variants	Comment	Organism
D43A	the mutant is unable to bind the Mg2+ cofactor	Thermotoga maritima
Y80A	the mutant is severely compromised in its ability to bind to DNA base lesions and the corresponding nicked product	Thermotoga maritima
Y80F	the mutant is similar to the wild type EndoV in its ability to bind to DNA base lesions and the corresponding nicked product	Thermotoga maritima
Y80H	the mutant is severely compromised in its ability to bind to DNA base lesions and the corresponding nicked product	Thermotoga maritima

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	contains one Mg2+ ion in the active site, which is required for activity	Thermotoga maritima

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	Q9X2H9	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap SP XL column chromatography	Thermotoga maritima

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA + H2O	EndoV hydrolyzes the second phosphodiester bond 3' of a deaminated base using Mg2+ as a cofactor	Thermotoga maritima	5'-phosphoryltermini DNA + 3'-hydroxyltermini DNA	-	?
additional information	EndoV cleaves substrates with base mismatches and helical distortions, such as mismatch loops, hairpins and flap structures, EndoV cleaves mismatched base pairs preferentially at adenine and guanine purines, EndoV binds to cleaved mismatch base pair products with much lower affinity as compared to cleaved deaminated bases	Thermotoga maritima	?	-	?

Synonyms

Synonyms	Comment	Organism
endonuclease V	-	Thermotoga maritima
EndoV	-	Thermotoga maritima

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)