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Literature summary for 3.1.21.7 extracted from

  • Gao, H.; Huang, J.; Barany, F.; Cao, W.
    Switching base preferences of mismatch cleavage in endonuclease V: an improved method for scanning point mutations (2007), Nucleic Acids Res., 35, e2/1-e2/6.
No PubMed abstract available
Search for more literature for 3.1.21.7

Activating Compound

Activating Compound Comment Organism Structure
additional information high pH, metal cofactor Mn2+, using excess enzyme, and/or solvents such as dimethyl sulfoxide and betaine, lead to cleavage of most mismatched DNA base pairs Thermotoga maritima

Protein Variants

Protein Variants Comment Organism
G41V mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
H125A mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
H214D mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
I81A mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
P207A mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
P209A mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
P82A mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
R118A mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
R205K mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
R211A mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
R211K mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
R88E mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
R88K mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
R88Q mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima
Y80A conversion to a C-specific mismatch cleavage variant Thermotoga maritima
Y80F mismatch cleavage activity similar to the wild-type enzyme Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ enhanced mismatch cleavage Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9X2H9
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O cleavage of deaminated bases at the second phosphodiester bond 3' downstream to a lesion Thermotoga maritima hydrolysed DNA
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 ?

Synonyms

Synonyms Comment Organism
endonuclease V
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 Thermotoga maritima