General Stability | Organism |
---|---|
unstable to freeze-thawing, unstable for long-term storage | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | strong inhibition | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | strong requirement, completely inactive if absent | Escherichia coli | |
Mn2+ | 15% of the Mg2+ enhanced activity at 2 mM | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
glycerol density gradient sedimentation | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded plasmid DNA + H2O | Escherichia coli | 10fold lower activity than for single-stranded circular DNA, relaxed plasmid DNA, exposure to UV light or sodium bisulfite, to pH 5.2 or osmium tetroxide renders duplex DNA 7fold more active, best substrate is uracil containing DNA from Bacillus subtilis | nicked circular DNA + linearized DNA | - |
? | |
single-stranded circular DNA + H2O | Escherichia coli | 10fold more activity than for duplex DNA | linearized single-stranded DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Escherichia coli | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.67 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, somewhat stable for several months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded plasmid DNA + H2O | 10fold lower activity than for single-stranded circular DNA, relaxed plasmid DNA, exposure to UV light or sodium bisulfite, to pH 5.2 or osmium tetroxide renders duplex DNA 7fold more active, best substrate is uracil containing DNA from Bacillus subtilis | Escherichia coli | nicked circular DNA + linearized DNA | - |
? | |
single-stranded circular DNA + H2O | 10fold more activity than for duplex DNA | Escherichia coli | linearized single-stranded DNA | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Escherichia coli |