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Literature summary for 3.1.21.7 extracted from

  • Nyaga, S.G.; Dodson, M.L.; Lloyd, R.S.
    Role of specific amino acid residues in T4 endonuclease V that alter nontarget DNA binding (1997), Biochemistry, 36, 4080-4088.
    View publication on PubMed
Search for more literature for 3.1.21.7

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Tequatrovirus T4

Protein Variants

Protein Variants Comment Organism
K121N decreased affinity for nontarget DNA Tequatrovirus T4
K86N decreased affinity for nontarget DNA Tequatrovirus T4
Q15R limited processive nicking activity Tequatrovirus T4
R117N limited processive nickin activity Tequatrovirus T4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 49-mer oligonucleotide mutant Q15R and R117N enzymes, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer Tequatrovirus T4
0.005
-
 49-mer oligonucleotide wild type enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer Tequatrovirus T4
0.013
-
 49-mer oligonucleotide mutant K121N enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer Tequatrovirus T4
0.024
-
 49-mer oligonucleotide mutant K86N enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Tequatrovirus T4 pyrimidine dimer-specific 5'-phosphoryltermini DNA + 3'-hydroxyltermini DNA
-
 ?
double-stranded plasmid DNA + H2O Tequatrovirus T4 supercoiled pBR322-DNA nicked circular DNA + linearized DNA
-
 ?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps, recombinant enzymes Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
12-mer oligonucleotide + H2O double-stranded, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer Tequatrovirus T4 hydrolyzed DNA
-
 ?
49-mer oligonucleotide + H2O double-stranded, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer Tequatrovirus T4 hydrolysed DNA
-
 ?
DNA + H2O pyrimidine dimer-specific Tequatrovirus T4 5'-phosphoryltermini DNA + 3'-hydroxyltermini DNA
-
 ?
double-stranded plasmid DNA + H2O supercoiled pBR322-DNA Tequatrovirus T4 nicked circular DNA + linearized DNA
-
 ?

Synonyms

Synonyms Comment Organism
T4 endonuclease V
-
 Tequatrovirus T4

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000333
-
 49-mer oligonucleotide mutant K121N enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer Tequatrovirus T4
0.00167
-
 49-mer oligonucleotide mutant R117N enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer Tequatrovirus T4
0.005
-
 49-mer oligonucleotide mutant Q15R enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer Tequatrovirus T4
0.00667
-
 49-mer oligonucleotide mutant K86N enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer Tequatrovirus T4
0.0117
-
 49-mer oligonucleotide wild type enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer Tequatrovirus T4