Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Tequatrovirus T4 |
Protein Variants | Comment | Organism |
---|---|---|
K121N | decreased affinity for nontarget DNA | Tequatrovirus T4 |
K86N | decreased affinity for nontarget DNA | Tequatrovirus T4 |
Q15R | limited processive nicking activity | Tequatrovirus T4 |
R117N | limited processive nickin activity | Tequatrovirus T4 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
49-mer oligonucleotide | mutant Q15R and R117N enzymes, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 | |
0.005 | - |
49-mer oligonucleotide | wild type enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 | |
0.013 | - |
49-mer oligonucleotide | mutant K121N enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 | |
0.024 | - |
49-mer oligonucleotide | mutant K86N enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Tequatrovirus T4 | pyrimidine dimer-specific | 5'-phosphoryltermini DNA + 3'-hydroxyltermini DNA | - |
? | |
double-stranded plasmid DNA + H2O | Tequatrovirus T4 | supercoiled pBR322-DNA | nicked circular DNA + linearized DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps, recombinant enzymes | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
12-mer oligonucleotide + H2O | double-stranded, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 | hydrolyzed DNA | - |
? | |
49-mer oligonucleotide + H2O | double-stranded, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 | hydrolysed DNA | - |
? | |
DNA + H2O | pyrimidine dimer-specific | Tequatrovirus T4 | 5'-phosphoryltermini DNA + 3'-hydroxyltermini DNA | - |
? | |
double-stranded plasmid DNA + H2O | supercoiled pBR322-DNA | Tequatrovirus T4 | nicked circular DNA + linearized DNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
T4 endonuclease V | - |
Tequatrovirus T4 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000333 | - |
49-mer oligonucleotide | mutant K121N enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 | |
0.00167 | - |
49-mer oligonucleotide | mutant R117N enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 | |
0.005 | - |
49-mer oligonucleotide | mutant Q15R enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 | |
0.00667 | - |
49-mer oligonucleotide | mutant K86N enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 | |
0.0117 | - |
49-mer oligonucleotide | wild type enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 |