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Literature summary for 3.1.21.6 extracted from
- Purification and characterization of a deoxyriboendonuclease from Mycobacterium smegmatis (2006), J. Biochem. Mol. Biol., 39, 140-144.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | deoxyribonuclease is an endonuclease, but is not a restriction endonuclease | Mycolicibacterium smegmatis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | chelates Mg2+, increasing Mg2+ concentrations can restore the activity of the enzyme | Mycolicibacterium smegmatis |  |
| K+ | - |
Mycolicibacterium smegmatis | |
| additional information | not inhibited by Na+ | Mycolicibacterium smegmatis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity, maximum activity at 2.5 mM | Mycolicibacterium smegmatis | |
| Mn2+ | can replace Mg2+ showing better activity | Mycolicibacterium smegmatis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
gel filtration | Mycolicibacterium smegmatis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium smegmatis | - |
mc26 culture | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to near homogeneity, 521fold by gel filtration | Mycolicibacterium smegmatis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 18 | - |
crude extract | Mycolicibacterium smegmatis |
| 9444 | - |
521fold purified enzyme | Mycolicibacterium smegmatis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, Tris-HCl buffer, pH 7.4, 10 mM EDTA, 10% glycerol, stable for at least 15 days | Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| covalently closed circular DNA pUC18 + H2O | - |
Mycolicibacterium smegmatis | ? | - |
? | |
| lambda DNA + H2O | - |
Mycolicibacterium smegmatis | ? | - |
? | |
| additional information | catalyzes the endonucleolytic cleavage of both the double- as well as the single-strand DNA non-specifically | Mycolicibacterium smegmatis | ? | - |
? | |
| pBR322 DNA + H2O | - |
Mycolicibacterium smegmatis | DNA fragments carrying phosphate groups at 5' ends and hydroxyl group at the 3' ends, respectively | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Deoxyriboendonuclease | - |
Mycolicibacterium smegmatis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 32 | - |
- |
Mycolicibacterium smegmatis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Mycolicibacterium smegmatis |
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