Literature summary for 3.1.21.5 extracted from

Moencke-Buchner, E.; Rothenberg, M.; Reich, S.; Wagenfuehr, K.; Matsumura, H.; Terauchi, R.; Krueger, D.H.; Reuter, M.
Functional characterization and modulation of the DNA cleavage efficiency of type III restriction endonuclease EcoP15I in its interaction with two sites in the DNA target (2009), J. Mol. Biol., 387, 1309-1319.

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General Stability

General Stability	Organism
EcoP15I is stabilized in the presence of specific DNA	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	sinefungin neither has an appreciable effect on DNA cleavage by EcoP15I nor compensated for the second recognition site; the catalytic activity of EcoP15I after 3 h of pre-incubation without DNA is strongly decreased	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA + H2O	EcoP15I rrequires the interaction with two inversely oriented 5'-CAGCAG recognition sites for efficient DNA cleavage	Escherichia coli	specific double-stranded DNA fragments with terminal 5'-phosphate	-	?
additional information	the pre-incubation of EcoP15I with DNA has no significant influence on the enzyme's ability to cleave DNA	Escherichia coli	?	-	?

Synonyms

Synonyms	Comment	Organism
EcoP15I	-	Escherichia coli
type III restriction endonuclease	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Escherichia coli
S-adenosyl-L-methionine	S-adenosyl-L-methionine is not necessary for DNA cleavage, the presence of S-adenosyl-L-methionine dramatically impaires DNA cleavage due to competing DNA methylation	Escherichia coli

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Release 2023.1 (January 2023)