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Literature summary for 3.1.21.5 extracted from

  • Neely, R.K.; Roberts, R.J.
    The BsaHI restriction-modification system: cloning, sequencing and analysis of conserved motifs (2008), BMC Mol. Biol., 9, 48.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain ER2566 Geobacillus stearothermophilus

Protein Variants

Protein Variants Comment Organism
D354A mutant shows reduced activity compared to the wild type enzyme Geobacillus stearothermophilus
E350A mutant shows reduced activity compared to the wild type enzyme Geobacillus stearothermophilus
F353A mutant shows reduced activity compared to the wild type enzyme Geobacillus stearothermophilus
P349A mutant shows reduced activity compared to the wild type enzyme Geobacillus stearothermophilus
Q344A mutant shows activity similar to wild type enzyme Geobacillus stearothermophilus
R351A the activity of the R351A mutant is negligible Geobacillus stearothermophilus
R352A mutant shows activity similar to the wild type enzyme Geobacillus stearothermophilus
S348A mutant shows activity similar to the wild type enzyme Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus B0LX59
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-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O BsaHI recognises a palindromic sequence of bases and cleaves within this sequence between the purine and cytosine bases: GR/CGYC Geobacillus stearothermophilus specific double-stranded DNA fragments with terminal 5'-phosphate
-
 ?
DNA + H2O BsaHI recognises a palindromic sequence of bases and cleaves within this sequence between the purine and cytosine bases: GR/CGYC Geobacillus stearothermophilus CPW11 specific double-stranded DNA fragments with terminal 5'-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
BsaHI
-
 Geobacillus stearothermophilus