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Literature summary for 3.1.21.5 extracted from

  • Janscak, P.; Sandmeier, U.; Szczelkun, M.D.; Bickle, T.A.
    Subunit assembly and mode of DNA cleavage of the type III restriction endonucleases EcoP1I and EcoP15I (2001), J. Mol. Biol., 306, 417-431.
    View publication on PubMed
Search for more literature for 3.1.21.5

Protein Variants

Protein Variants Comment Organism
D898A Res subunit, no enzymic activity Escherichia coli
D898A Res subunit, no enzymic activity prophage P1
E916A Res subunit, no enzymic activity Escherichia coli
E916A Res subunit, no enzymic activity prophage P1
K918A Res subunit, no enzymic activity Escherichia coli
K918A Res subunit, no enzymic activity prophage P1
P897A Res subunit, activity similar to wild type prophage P1
R534A Res subunit, no enzymic activity Escherichia coli
R534A Res subunit, no enzymic activity prophage P1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
 2 * 105000 + 2 * 74000, deduced from gene sequence Escherichia coli
74000
-
 2 * 105000 + 2 * 74000, deduced from gene sequence prophage P1
105000
-
 2 * 105000 + 2 * 74000, deduced from gene sequence Escherichia coli
105000
-
 2 * 105000 + 2 * 74000, deduced from gene sequence prophage P1
407000
-
 sedimentation studies Escherichia coli
409000
-
 sedimentation studies prophage P1

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 enzyme EcoP15I
-
prophage P1
-
 enzyme EcoP1I
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information cooperation of enzyme with EcoP15I in DNa cleavage, cleavage predominantly at EcoP15I site, EcpP1I greatly stimulates EcoP15I nicking activity Escherichia coli ?
-
 ?
additional information cooperation of enzyme with EcoP1I in DNa cleavage, cleavage predominantly at EcoP15I site, EcoP1I greatly stimulates EcoP15I nicking activity prophage P1 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 2 * 105000 + 2 * 74000, deduced from gene sequence Escherichia coli
tetramer 2 * 105000 + 2 * 74000, deduced from gene sequence prophage P1