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Literature summary for 3.1.21.4 extracted from

  • Zhang, Y.; Takaki, Y.; Yoshida-Takashima, Y.; Hiraoka, S.; Kurosawa, K.; Nunoura, T.; Takai, K.
    A sequential one-pot approach for rapid and convenient characterization of putative restriction-modification systems (2023), MSystems, 8, e0081723.
    View publication on PubMed
Search for more literature for 3.1.21.4

Application

Application Comment Organism
analysis sequential 1-day and one-pot workflow covering in vitro protein synthesis and enzymatic assays to confirm the exact function of putative restriction-modification systems. Fluorogenic molecular beacon probes containing all the probable recognition sequences, are designed and used for the fluorogenicenzyme assay Nitratiruptor phage NrS-3

Organism

Organism UniProt Comment Textmining
Nitratiruptor phage NrS-3 A0A7R7A8H4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA containing AGATCC + H2O
-
 Nitratiruptor phage NrS-3 ?
-
 ?
DNA containing GGATCT + H2O
-
 Nitratiruptor phage NrS-3 ?
-
 ?

Synonyms

Synonyms Comment Organism
NRS3_08
-
 Nitratiruptor phage NrS-3
R.Nph3I
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 Nitratiruptor phage NrS-3

General Information

General Information Comment Organism
physiological function REase R.Nph3I constitutes an active restriction-modification system with the adjacent putative MTase NRS3_07 and is sensitive to 6-methyl-adenosine DNA methylation Nitratiruptor phage NrS-3