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Literature summary for 3.1.21.4 extracted from
- The type IIS restriction enzyme MmeI can cut across a double-strand break (2023), Mol. Biol. Rep., 50, 5495-5499.
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylophilus methylotrophus | B2MU09 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | MmeI recognizes an asymmetric sequence TCCRAC and cuts 20 bp downstream | Methylophilus methylotrophus | ? | - |
- |
 |
| additional information | MmeI can cut across a double-strand break. MMeI shows78.5% digestion with a 2-base overhang, 96.6% with a 3-base overhang, and 90.6% with two sites on the same DNA molecule. A DNA molecule containing a 1-base overhang shows minimal digestion, and blunt-ended oligos with no complementary overhang produce no digestion at all | Methylophilus methylotrophus | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MmeI | - |
Methylophilus methylotrophus |
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