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Literature summary for 3.1.21.4 extracted from
- Assembly of complex pathways using type IIs restriction enzymes (2019), Methods Mol. Biol., 1927, 93-109.
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | protocol to quickly assemble multigene constructs using a series of simple one-pot assembly steps starting from libraries of cloned and sequenced parts. Basic parts consist of a DNA fragment of interest flanked by two BsaI restriction sites in opposite orientations cloned in a conventional cloning vector with a spectinomycin resistance cassette | Geobacillus stearothermophilus |
| molecular biology | protocol to quickly assemble multigene constructs using a series of simple one-pot assembly steps starting from libraries of cloned and sequenced parts | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | Q69B20 | isoform BsmBI | - |
| Geobacillus stearothermophilus | Q6SPF4 | isoform BsaI | - |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BsaI | - |
Geobacillus stearothermophilus |
| BsmBI | - |
Geobacillus stearothermophilus |
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