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Literature summary for 3.1.21.4 extracted from

  • Matsumura, I.
    Golden Gate assembly of BioBrick-compliant parts using Type II restriction endonucleases (2022), Biotechniques, 72, 185-193.
    View publication on PubMed
Search for more literature for 3.1.21.4

Application

Application Comment Organism
molecular biology DNA recombination methods employ commerciually available DNA methyltransferase expression vectors, that protect selected sites on different plasmids from particular Type II restriction endonucleases. One methods employs 4 restriction endonuclease and 2 methyltransferases in a discontinuous DNA assembly and produces more desired recombinant plasmids and produces fewer undesired recombination products as conventional subcloning. The 5 restriction endonucleases/methyltransferases continuous DNA assembly is approximately as efficient and specific as conventional Golden Gate assembly, even though in vivo methylation of one plasmid is incomplete Escherichia coli
molecular biology DNA recombination methods employ commercially available DNA methyltransferase expression vectors, that protect selected sites on different plasmids from particular Type II restriction endonucleases. One method employs 4 restriction endonuclease and 2 methyltransferases in a discontinuous DNA assembly and produces more desired recombinant plasmids and produces fewer undesired recombination products as conventional subcloning. The 5 restriction endonucleases/methyltransferases continuous DNA assembly is approximately as efficient and specific as conventional Golden Gate assembly, even though in vivo methylation of one plasmid is incomplete Streptomyces spectabilis

Organism

Organism UniProt Comment Textmining
Escherichia coli P00642 isoform EcoRI
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Streptomyces spectabilis A8WEY4 isoform SpeI
-