Literature summary for 3.1.21.4 extracted from

Sasnauskas, G.; Tamulaitiene, G.; Tamulaitis, G.; Calyseva, J.; Laime, M.; Rimsellene, R.; Lubys, A.; Siksnys, V.
UbaLAI is a monomeric Type IIE restriction enzyme (2017), Nucleic Acids Res., 45, 9583-9594.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method at 19°C. 1.6 A co-crystal structure of UbaLAI N-terminal domain	uncultured bacterium

Organism

Organism	UniProt	Comment	Textmining
uncultured bacterium	A0A291ATB4	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA + H2O	the enzyme is specific for the pseudosymmetric DNA sequence 5'-CC/WGG-3' (where W = A/T, and / marks the cleavage position). UbaLAI requires two recognition sites for optimal activity, and, like other type IIE enzymes, uses one copy of a recognition site to stimulate cleavage of a second copy. It is proposed that during the reaction the UbaLAI N-terminal domain acts as a handle that tethers the monomeric UbaLAI C-terminal domain to the DNA, thereby helping UbaLAI-C to perform two sequential DNA nicking reactions on the second recognition site during a single DNA-binding event	uncultured bacterium	?	-	?

Subunits

Subunits	Comment	Organism
monomer	-	uncultured bacterium

Synonyms

Synonyms	Comment	Organism
UbaLAI	-	uncultured bacterium

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Release 2026.1 (March 2026)