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Literature summary for 3.1.21.4 extracted from
- Type II restriction endonucleases-a historical perspective and more (2014), Nucleic Acids Res., 42, 7489-7527.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Escherichia coli |  |
| Mg2+ | required | Haemophilus influenzae | |
| Mn2+ | Mg2+ can be replaced by Mn2+ albeit with lower efficiency | Escherichia coli | |
| Mn2+ | Mg2+ can be replaced by Mn2+ albeit with lower efficiency | Haemophilus influenzae | |
| additional information | not activated by Ca2+ and Na+ | Escherichia coli | |
| additional information | not activated by Ca2+ and Na+ | Haemophilus influenzae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
x * 30000, EcoRI, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | Escherichia coli | cleavage by EcoRI is staggered, producing fragments with 4-nucleotide single-stranded overhangs, recognition sequence is GAATTC | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| double-stranded DNA + H2O | Escherichia coli | cleavage by EcoRV is staggered, producing fragments with 4-nucleotide single-stranded overhangs, recognition sequence is GATATC | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| double-stranded DNA + H2O | Haemophilus influenzae | cleavage by HindII is blunt, producing fragments with flush ends | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Haemophilus influenzae | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | cleavage by EcoRI is staggered, producing fragments with 4-nucleotide single-stranded overhangs, recognition sequence is GAATTC | Escherichia coli | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| double-stranded DNA + H2O | cleavage by EcoRV is staggered, producing fragments with 4-nucleotide single-stranded overhangs, recognition sequence is GATATC | Escherichia coli | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| double-stranded DNA + H2O | cleavage by HindII is blunt, producing fragments with flush ends | Haemophilus influenzae | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | x * 30000, EcoRI, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EcoRI | - |
Escherichia coli |
| EcoRV | - |
Escherichia coli |
| HindII | - |
Haemophilus influenzae |
| type II REase | - |
Escherichia coli |
| type II REase | - |
Haemophilus influenzae |
| type II restriction endonuclease | - |
Escherichia coli |
| type II restriction endonuclease | - |
Haemophilus influenzae |
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