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Literature summary for 3.1.21.4 extracted from

  • Smith, R.M.; Pernstich, C.; Halford, S.E.
    TstI, a Type II restriction-modification protein with DNA recognition, cleavage and methylation functions in a single polypeptide (2014), Nucleic Acids Res., 42, 5809-5822.
    View publication on PubMed
Search for more literature for 3.1.21.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli T7 Express cells Thermus scotoductus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
594000
-
 His-tagged enzyme, gel filtration Thermus scotoductus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded DNA + H2O Thermus scotoductus
-
 double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
double-stranded DNA + H2O Thermus scotoductus RFL1
-
 double-stranded DNA fragments with terminal 5'-phosphates
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus scotoductus
-
-
-
Thermus scotoductus RFL1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded DNA + H2O
-
 Thermus scotoductus double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
double-stranded DNA + H2O
-
 Thermus scotoductus RFL1 double-stranded DNA fragments with terminal 5'-phosphates
-
 ?

Subunits

Subunits Comment Organism
homotetramer
-
 Thermus scotoductus

Synonyms

Synonyms Comment Organism
REase
-
 Thermus scotoductus
TstI
-
 Thermus scotoductus
type II restriction-modification protein
-
 Thermus scotoductus

Cofactor

Cofactor Comment Organism Structure
additional information does not need S-adenosyl-L-methionine as cofactor Thermus scotoductus