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Literature summary for 3.1.21.4 extracted from

  • Podgorska, B.; Kujawska, G.; Skurzewski, M.; Batsko, O.; Kaczorowski, T.
    A rapid and simple method for detection of type II restriction endonucleases in cells of bacteria with high activity of nonspecific nucleases (2012), Acta Biochim. Pol., 59, 669-672.
    View publication on PubMed
Search for more literature for 3.1.21.4

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Citrobacter freundii
Mg2+ required Escherichia coli
Mg2+ required Klebsiella pneumoniae
Mg2+ required Pseudescherichia vulneris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded DNA + H2O Klebsiella pneumoniae
-
 double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
double-stranded DNA + H2O Escherichia coli
-
 double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
double-stranded DNA + H2O Pseudescherichia vulneris
-
 double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
double-stranded DNA + H2O Citrobacter freundii
-
 double-stranded DNA fragments with terminal 5'-phosphates
-
 ?

Organism

Organism UniProt Comment Textmining
Citrobacter freundii
-
-
-
Escherichia coli
-
-
-
Klebsiella pneumoniae
-
-
-
Pseudescherichia vulneris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
phosphocellulose P-11 column chromatography, heparin Sepharose column chromatography, CM Sephadex C-50 gel filtration or Blue-agarose column chromatography Citrobacter freundii
phosphocellulose P-11 column chromatography, heparin Sepharose column chromatography, CM Sephadex C-50 gel filtration or Blue-agarose column chromatography Escherichia coli
phosphocellulose P-11 column chromatography, heparin Sepharose column chromatography, CM Sephadex C-50 gel filtration or Blue-agarose column chromatography Klebsiella pneumoniae
phosphocellulose P-11 column chromatography, heparin Sepharose column chromatography, CM Sephadex C-50 gel filtration or Blue-agarose column chromatography Pseudescherichia vulneris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded DNA + H2O
-
 Klebsiella pneumoniae double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
double-stranded DNA + H2O
-
 Escherichia coli double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
double-stranded DNA + H2O
-
 Pseudescherichia vulneris double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
double-stranded DNA + H2O
-
 Citrobacter freundii double-stranded DNA fragments with terminal 5'-phosphates
-
 ?

Synonyms

Synonyms Comment Organism
BspI
-
 Citrobacter freundii
BstII
-
 Klebsiella pneumoniae
EcoRV
-
 Escherichia coli
EcoVIII
-
 Pseudescherichia vulneris
site-specific type II endonuclease
-
 Klebsiella pneumoniae
site-specific type II endonuclease
-
 Escherichia coli
site-specific type II endonuclease
-
 Pseudescherichia vulneris
site-specific type II endonuclease
-
 Citrobacter freundii
type II REase
-
 Klebsiella pneumoniae
type II REase
-
 Escherichia coli
type II REase
-
 Pseudescherichia vulneris
type II REase
-
 Citrobacter freundii
type II restriction enzyme
-
 Klebsiella pneumoniae
type II restriction enzyme
-
 Escherichia coli
type II restriction enzyme
-
 Pseudescherichia vulneris
type II restriction enzyme
-
 Citrobacter freundii