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Literature summary for 3.1.21.4 extracted from

  • Rasko, T.; Der, A.; Klement, E.; Slaska-Kiss, K.; Posfai, E.; Medzihradszky, K.F.; Marshak, D.R.; Roberts, R.J.; Kiss, A.
    BspRI restriction endonuclease: cloning, expression in Escherichia coli and sequential cleavage mechanism (2010), Nucleic Acids Res., 38, 7155-7166.
    View publication on PubMed
Search for more literature for 3.1.21.4

Cloned(Commentary)

Cloned (Comment) Organism
gene bspRIR, cloning from R genomic DNA, expression in Escherichia coli, which is dependent on the replacement of the native TTG initiation codon with an ATG codon, method development, overview Lysinibacillus sphaericus

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus
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 gene bspRIR
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information usage of a plasmid containing a single BspRI recognition site to analyze kinetically nicking and second-strand cleavage under steady-state conditions. Cleavage of the supercoiled plasmid goes through a relaxed intermediate indicating sequential hydrolysis of the two strands. BspRI cleaves the two DNA strands sequentially Lysinibacillus sphaericus ?
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 ?

Subunits

Subunits Comment Organism
additional information peptide mapping, tryptic digestion and mass spectrometry analysis, overview Lysinibacillus sphaericus

Synonyms

Synonyms Comment Organism
BspRI
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 Lysinibacillus sphaericus
GGCC-specific restriction endonuclease
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 Lysinibacillus sphaericus
additional information BspRI and the related putative restriction endonucleases belong to the PD-(D/E)XK nuclease superfamily Lysinibacillus sphaericus
type IIP REase
-
 Lysinibacillus sphaericus
type IIP restriction endonuclease
-
 Lysinibacillus sphaericus

General Information

General Information Comment Organism
additional information type IIP restriction endonucleases are characterized by recognition sequences displaying dyad axes of symmetry (palindromes), and constitute the most abundant class of characterized restriction enzymes Lysinibacillus sphaericus