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Literature summary for 3.1.21.4 extracted from
- Solution parameters modulating DNA binding specificity of the restriction endonuclease EcoRV (2011), FEBS J., 278, 2713-2727.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of EcoRV-DNA binding, detailed overview. Slow kinetics of complex formation at pH 7.6 | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Escherichia coli | |
| additional information | the salt dependence of Knsp-sp for EcoRV-DNA binding, kinetics, overview | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | development of a self-cleavage assay to measure EcoRV-DNA competitive binding and to evaluate the influence of water activity, pH and salt concentration on the DNA substrate binding stringency of the enzyme in the absence of divalent ions. The enzyme can readily distinguish specific and nonspecific sequences. The relative specific-nonspecific binding constant increases strongly with increasing neutral solute concentration and with decreasing pH. In addition to divalent ions, water activity and pH are key parameters that strongly modulate binding specificity of EcoRV | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EcoRV | - |
Escherichia coli |
| restriction endonuclease EcoRV | - |
Escherichia coli |
| type II restriction endonuclease | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | strong pH dependence of the specific-nonspecific association binding constant ratio, increasing about 500fold between pH 8.0 and pH 5.5, overview | Escherichia coli |
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