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Literature summary for 3.1.21.4 extracted from

  • Kachalova, G.S.; Rogulin, E.A.; Yunusova, A.K.; Artyukh, R.I.; Perevyazova, T.A.; Matvienko, N.I.; Zheleznaya, L.A.; Bartunik, H.D.
    Structural analysis of the heterodimeric type IIS restriction endonuclease R.BspD6I acting as a complex between a monomeric site-specific nickase and a catalytic subunit (2008), J. Mol. Biol., 384, 489-502.
    View publication on PubMed
Search for more literature for 3.1.21.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Bacillus sp. (in: firmicutes)

Crystallization (Commentary)

Crystallization (Comment) Organism
small and large subunit of Bsp6I Bacillus sp. (in: firmicutes)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70800
-
 1 * 70800 + 1 * ?, X-ray crystallography Bacillus sp. (in: firmicutes)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: firmicutes)
-
-
-
Bacillus sp. (in: firmicutes) D6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: firmicutes)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O BspD6I cleaves both DNA strands within the recognition sequence Bacillus sp. (in: firmicutes) double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
DNA + H2O BspD6I cleaves both DNA strands within the recognition sequence Bacillus sp. (in: firmicutes) D6 double-stranded DNA fragments with terminal 5'-phosphates
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 70800 + 1 * ?, X-ray crystallography Bacillus sp. (in: firmicutes)

Synonyms

Synonyms Comment Organism
BspD6I
-
 Bacillus sp. (in: firmicutes)
Nt.BspD6I large subunit of Bsp6DI Bacillus sp. (in: firmicutes)
ss.BspD6I small subunit of Bsp6DI Bacillus sp. (in: firmicutes)
type II REase
-
 Bacillus sp. (in: firmicutes)
type IIS restriction endonuclease
-
 Bacillus sp. (in: firmicutes)