Literature summary for 3.1.21.4 extracted from

Hong, S.P.; Ji, S.I.; Rhee, H.; Shin, S.K.; Hwang, S.Y.; Lee, S.H.; Lee, S.D.; Oh, H.B.; Yoo, W.; Kim, S.O.
A simple and accurate SNP scoring strategy based on typeIIS restriction endonuclease cleavage and matrix-assisted laser desorption/ionization mass spectrometry (2008), BMC Genomics, 9, 276.

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Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	-	-	-
Geobacillus stearothermophilus F5	-	-	-
Planomicrobium okeanokoites	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA + H2O	BstF5I cleaves DNA 2 bases 3' to the recognition site on one strand and immediately 3' to the recognition site on the opposite strand, leaving a two base overhang	Geobacillus stearothermophilus	double-stranded DNA fragments with terminal 5'-phosphates	-	?
DNA + H2O	the FokI enzyme cleaves DNA 9 bases 3' to the recognition site on one strand and 13 bases from the recognition site on the other strand, leaving a four base overhang protruding 5' end	Planomicrobium okeanokoites	double-stranded DNA fragments with terminal 5'-phosphates	-	?
DNA + H2O	BstF5I cleaves DNA 2 bases 3' to the recognition site on one strand and immediately 3' to the recognition site on the opposite strand, leaving a two base overhang	Geobacillus stearothermophilus F5	double-stranded DNA fragments with terminal 5'-phosphates	-	?

Synonyms

Synonyms	Comment	Organism
BstF5I	-	Geobacillus stearothermophilus
FokI	-	Planomicrobium okeanokoites
typeIIS restriction endonuclease	-	Geobacillus stearothermophilus
typeIIS restriction endonuclease	-	Planomicrobium okeanokoites

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Release 2023.1 (January 2023)