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Literature summary for 3.1.21.4 extracted from

  • Doyon, J.B.; Pattanayak, V.; Meyer, C.B.; Liu, D.R.
    Directed evolution and substrate specificity profile of homing endonuclease I-SceI (2006), J. Am. Chem. Soc., 128, 2477-2484.
    View publication on PubMed
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Application

Application Comment Organism
biotechnology evolvement of mutant enzymes with altered DNA cleavage specificities by application of an in vivo positive and negative selection system that applies evolutionary pressure either to favor the cleavage of a desired target sequence or to disfavor the cleavage of a nontarget sequence Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information evolvement of mutant enzymes with altered DNA cleavage specificities by application of an in vivo positive and negative selection system that applies evolutionary pressure either to favor the cleavage of a desired target sequence or to disfavor the cleavage of a nontarget sequence Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 isoform Sce-I
-