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Literature summary for 3.1.21.4 extracted from
- Crystal structures of type II restriction endonuclease EcoO109I and its complex with cognate DNA (2005), J. Biol. Chem., 280, 5605-5610.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystal structure of EcoO109I and its complex with DNA | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | one metal ion and two water molecules are observed near the active site of the DNA complex. The metal ion is a Lewis acid that stabilizes the pentavalent phosphorus atom in the transition state. One water molecule, activated by Lys126, attacks the phosphorous atom in an SN2 mechanism, whereas the other water interacts with the 3'-leaving oxygen to donnate a proton to the oxygen | Escherichia coli | double-stranded DNA fragments with terminal 5'-phosphates | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| type II restriction endonuclease EcoO109I | - |
Escherichia coli |
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