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Literature summary for 3.1.21.4 extracted from

  • Armalyte, E.; Bujnicki, J.M.; Giedriene, J.; Gasiunas, G.; Kosinski, J.; Lubys, A.
    Mva1269I: a monomeric type IIS restriction endonuclease from Micrococcus varians with two EcoRI- and FokI-like catalytic domains (2005), J. Biol. Chem., 280, 41584-41594.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Kocuria varians Q2QHU9
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O Mva1269I restriction endonuclease recognizes an asymmetric DNA sequence 5'-GAATGCN-/-3'/5'-NG-/-CATTC-3' and cuts top and bottom DNA strands. The enzyme possesses two active sites responsible for the sequential cleavage of each DNA strand, which has evolved by fusion of a sequence specific nuclease domain, similar to EcoRI, to a nonspecific nuclease domain, similar to FokI Kocuria varians double-stranded DNA fragments with terminal 5'-phosphates
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Subunits

Subunits Comment Organism
monomer the enzyme possesses two active sites responsible for the sequential cleavage of each DNA strand, which has evolved by fusion of a sequence specific nuclease domain, similar to EcoRI, to a nonspecific nuclease domain, similar to FokI Kocuria varians

Synonyms

Synonyms Comment Organism
Mva1269I
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Kocuria varians
Mva1269I restriction endonuclease
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Kocuria varians
type IIS restriction endonuclease
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Kocuria varians