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Literature summary for 3.1.21.4 extracted from
- Eco1524I, a type II restriction endonuclease: isolation, partial purification, and characterization (2005), Appl. Biochem. Biotechnol., 125, 189-199.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Escherichia coli |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | Eco1524I recognizes the sequence 6-bp palindromic 5'AGG downward arrow CCT3', producing blunt end | Escherichia coli | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Eco1524I | - |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
stable up to | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | active from pH 6.0 to pH 10.0 | Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | stable from pH 6.0 to pH 10.0 | Escherichia coli |
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