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Literature summary for 3.1.21.4 extracted from

  • Zhu, Z.; Samuelson, J.C.; Zhou, J.; Dore, A.; Xu, S.Y.
    Engineering strand-specific DNA nicking enzymes from the type IIS restriction endonucleases BsaI, BsmBI, and BsmAI (2004), J. Mol. Biol., 337, 573-583.
    View publication on PubMed
Search for more literature for 3.1.21.4

Protein Variants

Protein Variants Comment Organism
DELTA362-465 nicking variant of BsaI, nicking occurs on top strand Geobacillus stearothermophilus
DELTA440-544 nicking variant of BsaI, nicking occurs on bottom strand Geobacillus stearothermophilus
DELTA446-544 nicking variant of BsaI Geobacillus stearothermophilus
K150R/R236G nicking variant of BsaI, nicking occurs on top strand Geobacillus stearothermophilus
N349Y nicking variant of BsmAI, nicking occurs on top strand Geobacillus stearothermophilus
N415D/R416G variant of BsmAI, both nicking activity and dsDNA cleavage Geobacillus stearothermophilus
N441D variant of BsaI, cleavage of dsDNA Geobacillus stearothermophilus
N441D/R442G nicking variant of BsaI, nicking occurs on bottom strand Geobacillus stearothermophilus
R221D nicking variant of BsmAI, nicking on top strand Geobacillus stearothermophilus
R233D nicking variant of BsmBI, nicking on top strand, about 5-10% cleavage of dsDNA Geobacillus stearothermophilus
R236D nicking variant of BsaI, nicking on top strand, no cleavage of dsDNA Geobacillus stearothermophilus
R236G nicking variant of BsaI, 5-10% cleavage of dsDNA Geobacillus stearothermophilus
R386S nicking variant of BsaI, nicking occurs on top strand Geobacillus stearothermophilus
R438D nicking variant of BsmBI, nicking on bottom strand Geobacillus stearothermophilus
R438G nicking variant of BsmBI Geobacillus stearothermophilus
R442G nicking variant of BsaI, 5% cleavage of dsDNA Geobacillus stearothermophilus
S128L/R236G nicking variant of BsaI, nicking occurs on top strand Geobacillus stearothermophilus
W8C/G207E nicking variant of BsaI, nicking occurs on top strand Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
 enzymes BsaI, BsmBI, BsmAI
-