Any feedback?
Literature summary for 3.1.21.4 extracted from
- Biochemical characterization of the restriction-modification system of Bacillus sphaericus (1978), Eur. J. Biochem., 89, 523-529.
General Stability
| General Stability | Organism |
|---|---|
| in dilute solutions at 37°C, 50% inactivation after 30 min | Lysinibacillus sphaericus |
| inactivation by lyophilization | Lysinibacillus sphaericus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| high ionic strength | irreversible inactivation | Lysinibacillus sphaericus | |
| Zn2+ | 2 mM, complete inhibition | Lysinibacillus sphaericus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Lysinibacillus sphaericus | |
| Mn2+ | can partially replace Mg2+, optimal concentration is 10 mM | Lysinibacillus sphaericus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
gel filtration | Lysinibacillus sphaericus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lysinibacillus sphaericus | - |
enzyme endoR*Bsp | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lysinibacillus sphaericus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% glycerol, 8-12 months no appreciable loss of activity | Lysinibacillus sphaericus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | single strandede DNA and double stranded DNA | Lysinibacillus sphaericus | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 35000, SDS-PAGE | Lysinibacillus sphaericus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endoR*Bsp | - |
Lysinibacillus sphaericus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
- |
Lysinibacillus sphaericus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | some activity is detected even at pH 5 and pH 10 | Lysinibacillus sphaericus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
