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Literature summary for 3.1.21.4 extracted from

  • Koncz, C.; Kiss, A.; Venetianer, P.
    Biochemical characterization of the restriction-modification system of Bacillus sphaericus (1978), Eur. J. Biochem., 89, 523-529.
    View publication on PubMed

General Stability

General Stability Organism
in dilute solutions at 37°C, 50% inactivation after 30 min Lysinibacillus sphaericus
inactivation by lyophilization Lysinibacillus sphaericus

Inhibitors

Inhibitors Comment Organism Structure
high ionic strength irreversible inactivation Lysinibacillus sphaericus
Zn2+ 2 mM, complete inhibition Lysinibacillus sphaericus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Lysinibacillus sphaericus
Mn2+ can partially replace Mg2+, optimal concentration is 10 mM Lysinibacillus sphaericus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
gel filtration Lysinibacillus sphaericus

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus
-
enzyme endoR*Bsp
-

Purification (Commentary)

Purification (Comment) Organism
-
Lysinibacillus sphaericus

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, 8-12 months no appreciable loss of activity Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O single strandede DNA and double stranded DNA Lysinibacillus sphaericus double-stranded DNA fragments with terminal 5'-phosphates
-
?

Subunits

Subunits Comment Organism
monomer 1 * 35000, SDS-PAGE Lysinibacillus sphaericus

Synonyms

Synonyms Comment Organism
endoR*Bsp
-
Lysinibacillus sphaericus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
Lysinibacillus sphaericus

pH Range

pH Minimum pH Maximum Comment Organism
5 10 some activity is detected even at pH 5 and pH 10 Lysinibacillus sphaericus