Cloned (Comment) | Organism |
---|---|
pHluorin-assisted expression of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
sitting-drop vapour-diffusion technique, crystallization and X-ray diffraction data analysis of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I. The shortest of the three encompassed HsdR residues 887-1038 and yielded crystals that belonged to the orthorhombic space group C2221, with unit-cell parameters a = 83.42, b = 176.58, c = 126.03 A, alpha = beta = gamma = 90.0 and two molecules in the asymmetric unit. X-ray diffraction data are collected to a resolution of 2.45 A | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | Q304R3 | HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I | - |
Purification (Comment) | Organism |
---|---|
pHluorin-assisted purification of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I | Escherichia coli |
Subunits | Comment | Organism |
---|---|---|
More | the HsdR subunit of the type I restriction-modification system EcoR124I is responsible for the translocation as well as the restriction activity of the whole complex consisting of the HsdR, HsdM and HsdS subunits | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
type I restriction-modification system EcoR124I | - |
Escherichia coli |