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Literature summary for 3.1.21.3 extracted from

  • Youell, J.; Firman, K.
    EcoR124I: from plasmid-encoded restriction-modification system to nanodevice (2008), Microbiol. Mol. Biol. Rev., 72, 365-77.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.3

Activating Compound

Activating Compound Comment Organism Structure
S-adenosyl methionine stimulates DNA cleavage activity Escherichia coli

Application

Application Comment Organism
biotechnology potential uses for EcoR124I as a nanoactuator within a biosensor in the field of bionanotechnology. It may be used as a molecular dynamo that can measure events from single molecules of DNA, providing a highly sensitive biosensor for detecting events that interrupt translocation events Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli
recombinant plasmid pJS4M overproducing HsdM compared to HsdS Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information point mutation within the hsdS gene or insertional mutagenesis using the Tn5 transposon, both show new DNA specificities. Both new specificities are due to the production of a truncated HsdS subunit, which is able to dimerize, producing the recognition sequence GAAn7TTC in the case of the point mutation of EcoR124I, which produces a stop codon and hence the truncated subunit Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane the EcoR124I holoenzyme is particularly exposed on the periplasmic side of the cytoplasmic membrane Escherichia coli 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 116000, HsdR, x * 55000, HsdM, x * 43000, HsdS Escherichia coli
55000
-
 x * 116000, HsdR, x * 55000, HsdM, x * 43000, HsdS Escherichia coli
116000
-
 x * 116000, HsdR, x * 55000, HsdM, x * 43000, HsdS Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O HsdR subunit can produce only a single cleavage of the phosphodiester backbone of the DNA but can cooperate with another HsdR subunit to produce full DNA cleavage, producing DNA with overhanging ends of single-stranded DNA. On a single-site plasmid, cleavage requires the association of HsdR, from solution, to the cleavage complex in order to produce double-strand cleavage Escherichia coli ?
-
 ?
duplex DNA + ATP
-
 Escherichia coli double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 116000, HsdR, x * 55000, HsdM, x * 43000, HsdS Escherichia coli

Synonyms

Synonyms Comment Organism
EcoR124I
-
 Escherichia coli
type I R-M enzyme
-
 Escherichia coli
type I restriction-modification enzyme
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli
ATP required Escherichia coli
S-adenosyl-L-methionine activity is stimulated by S-adenosyl-L-methionine Escherichia coli