Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strains BNH670 or GM31 harboring a plasmid with various versions of the EcoRII RM gene complex | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
L80P | L80P mutation in the modification enzyme of the EcoRII gene complex confers thermosensitivity of cell growth (shows activity at 30°C but not at 37°C). Under a condition of inhibited protein synthesis, the activity of the mutant is completely lost at a high temperature. In parallel, the L80P mutant protein disappears more rapidly than the wild-type protein | Escherichia coli |
additional information | N-terminal 83-amino-acid deletion mutant, shows activity at at 30°C and 37°C. The EcoRII RM gene complex is a mutant carrying a T239C (L80P) substitution in the modification gene and a synonymous T402C substitution in the restriction gene. The capability of the restriction-modification system EcoRII in forcing maintenance on its host can be modulated by a region of its antitoxin, the modification enzyme, as in the classical postsegregational killing systems | Escherichia coli |
T239C | shows decreased DNA methyltransferase activity at a higher temperature in vivo and in vitro than the nonmutated enzyme | Escherichia coli |
T402C | cells carrying the mutation are able to grow at 42°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
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Escherichia coli | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
EcoRII modification enzyme | - |
Escherichia coli |
EcoRII RM gene complex | - |
Escherichia coli |
EcoRII system | - |
Escherichia coli |