Cloned (Comment) | Organism |
---|---|
expression of GST-Endo IV fusion protein in Escherichia coli: wild-type and mutants W88R and S176N | Tequatrovirus T4 |
Protein Variants | Comment | Organism |
---|---|---|
S176N | mutant enzyme retains cleavage activity (17.5% of that of wild-type Endo IV), but loses the polarized and restricted cleavage of a dCs tract. Escherichia coli cells expressing the intact Endo IV mutant enzyme are viable and, in contrast to wild-type Endo IV, the mutant enzyme does not show detrimental effect on the host cells | Tequatrovirus T4 |
W88R | mutant enzyme shows no enzymatic activity (less than 0.4% of that of wild-type Endo IV). Escherichia coli cells expressing the intact Endo IV mutant enzyme are viable and, in contrast to wild-type Endo IV, these mutant enzymes do not show detrimental effect on the host cells | Tequatrovirus T4 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-values of the wild-type and S176N mutant forms of Endo IV are determined with series of 25-base oligonucleotides | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
single-stranded DNA + H2O | Tequatrovirus T4 | the Ser176 of T4 endonuclease IV is crucial for the restricted and polarized dC-specific cleavage of single-stranded DNA implicated in restriction of dC-containing DNA in host Escherichia coli | 5'-phosphooligonucleotides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
GST-Endo IV fusion proteins: wild-type and mutants W88R and S176N | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
single-stranded DNA + H2O | the Ser176 of T4 endonuclease IV is crucial for the restricted and polarized dC-specific cleavage of single-stranded DNA implicated in restriction of dC-containing DNA in host Escherichia coli | Tequatrovirus T4 | 5'-phosphooligonucleotides | - |
? | |
single-stranded DNA + H2O | endonuclease IV cleaves single-stranded (ss) DNA in a dC-specific manner. The Ser176 of T4 endonuclease IV is crucial for the restricted and polarized dC-specific cleavage of single-stranded DNA implicated in restriction of dC-containing DNA in host Escherichia coli | Tequatrovirus T4 | 5'-phosphooligonucleotides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endonuclease IV | - |
Tequatrovirus T4 |