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Literature summary for 3.1.21.2 extracted from

  • Friedberg, E.C.; Hadi, S.M.; Goldthwait, D.A.
    Endonuclease II of Escherichia coli. II. Enzyme properties and studies on the degradation of alkylated and native deoxyribonucleic acid (1969), J. Biol. Chem., 244, 5879-5889.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin 130% activity at 1% at pH 8.0 and 37°C Escherichia coli
additional information the enzyme is induced by phage T4, no activation by S-adenosyl-L-methionine, ATP, or both Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information analysis of enzyme activity in naturally occurring mutants of Escherichia coli compared to wild-type strain enzymes, overview Escherichia coli

General Stability

General Stability Organism
glycerol and bovine serum albumin stabilize Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
4-Chloromercuriphenylsulfonate
-
Escherichia coli
8-hydroxyquinoline inhibits cleavage of alkylated dsDNA Escherichia coli
Co2+
-
Escherichia coli
diethyldithiocarbamic acid
-
Escherichia coli
EDTA inhibits cleavage of non-alkylated dsDNA, 70% inhibition at 0.1 mM Escherichia coli
KCl 24% inhibition at 0.1 M, 42% at 0.2 M, and 64% at 0.3 M Escherichia coli
additional information enzyme inhibition by chelating and sulfhydryl agents, but not by tRNA and caffeine, DTT or 2-mercaptoethanol protect against inhibition by sulfhydryl reagents Escherichia coli
Zn2+
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stimulates Escherichia coli
Mn2+ stimulates Escherichia coli
additional information the enzyme shows no absolute requirement for divalent cations, no effect by Ca2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
sucrose density gradient sedimentation equilibrium analysis Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded alkylated DNA + H2O Escherichia coli endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides 5'-phosphooligonucleotides
-
ir
double-stranded nativeDNA + H2O Escherichia coli endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides 5'-phosphooligonucleotides
-
ir

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
several strains
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
Endonucleolytic cleavage of ssDNA at apurinic/apyrimidinic sites to 5'-phosphooligonucleotide end-products reaction mechanism Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
relative activity in several Escherichia coli wild-type and mutant strains, overview Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, purified enzyme, 40 mM potassium phosphate, pH 6.5, or 50 mM Tris-HCl, pH 8.0, with 0.1 mM DTT or 2-mercaptoethanol, moderately stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded alkylated DNA + H2O endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides Escherichia coli 5'-phosphooligonucleotides
-
ir
double-stranded alkylated DNA + H2O endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides, hydrolysis of a phosphodiester bond near an alkylated base in native DNA without single breaks in the region, the enzyme makes predominantly single-strand break, and a few double-strand breaks, the ratio is 3.7:1 Escherichia coli 5'-phosphooligonucleotides
-
ir
double-stranded native DNA + H2O endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides, the enzyme makes predominantly single-strand break, and a few double-strand breaks, the ratio is 3.7:1 Escherichia coli 5'-phosphooligonucleotides
-
ir
double-stranded nativeDNA + H2O endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides Escherichia coli 5'-phosphooligonucleotides
-
ir
additional information DNA substrate specificity, overview Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
endonuclease II
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
loss of 41% activity after 30 min, loss of 92% activity after 2 h, with addition 50% glycerol only 6% activity is lost within 2 h Escherichia coli
45
-
10 min, fraction IV, loss of 10% activity Escherichia coli
60
-
10 min, fraction IV, complete inactivation Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
Escherichia coli