Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | reduces activity 10fold at a concentration of 200 mM | Escherichia coli | |
potassium glutamate | reduces activity 10fold at a concentration of 200 mM | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | poor substitute for Mg2+ | Escherichia coli | |
Co2+ | at 25 mM moderately effective for activity | Escherichia coli | |
Mg2+ | optimum: 8-25 mM, binding per se is independent of Mg2+, cleavage mechanism depends on Mg2+ | Escherichia coli | |
Mg2+ | required for activation | Escherichia coli | |
Mn2+ | can substitute for Mg2+, but is required in higher amounts | Escherichia coli | |
Zn2+ | poor substitute for Mg2+ | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Escherichia coli | holliday structure | hydrolyzed DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | nicks the ssDNA strands across the junction at symmetrical positions within the homologous arms | Escherichia coli | hydrolyzed DNA | - |
? | |
DNA + H2O | sequence specific: 5'- ATT - cleavage site - G-3', substrate: homologous core of 12 bp between the arms is required for cleavage but not for binding of the enzyme to the substrate, cleaves also Y-junctions with homologous core | Escherichia coli | hydrolyzed DNA | - |
? | |
DNA + H2O | holliday structure | Escherichia coli | hydrolyzed DNA | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Escherichia coli |