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Literature summary for 3.1.21.1 extracted from
- Putative secondary active site of bovine pancreatic deoxyribonuclease I (2008), Protein Pept. Lett., 15, 640-646.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | His44 may play a critical role in substrate DNA binding in the putative secondary active site, and introduction of sulfhydryl groups at Thr14 and Ser43 may facilitate Mn2+-coordination and further contribute to the catalytic activity of DNase I | Bos taurus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into vector pET15b and expressed in Escherichia coli strain BL21(DE3)pLysE | Bos taurus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H44A | DNase I activity is similar to that of the wild-type | Bos taurus |
| H44D | inactive, can only cleave the Mn2+-DNA substrate in a single nicking mode | Bos taurus |
| S43A | in the presence of Mn2+, as the wild-type, is able to hydrolyze the Mn2+-substrate forming-linear duplex DNA in addition to the relaxed open-circular DNA, indicating double scission | Bos taurus |
| S43A/H44D | inactive, can only cleave the Mn2+-DNA substrate in a single nicking mode | Bos taurus |
| S43C | in the presence of Mn2+, as the wild-type, is able to hydrolyze the Mn2+-substrate forming-linear duplex DNA in addition to the relaxed open-circular DNA, indicating double scission | Bos taurus |
| T14A | in the presence of Mn2+, as the wild-type, is able to hydrolyze the Mn2+-substrate forming-linear duplex DNA in addition to the relaxed open-circular DNA, indicating double scission | Bos taurus |
| T14A/H44D | inactive, can only cleave the Mn2+-DNA substrate in a single nicking mode | Bos taurus |
| T14A/S43A | in the presence of Mn2+, though being less active than the wild-type, can still cleave the plasmid DNA in the double scission mode | Bos taurus |
| T14A/S43A/H44D | inactive | Bos taurus |
| T14A/S43C | DNase I activity is similar to that of the wild-type | Bos taurus |
| T14C | in the presence of Mn2+, as the wild-type, is able to hydrolyze the Mn2+-substrate forming-linear duplex DNA in addition to the relaxed open-circular DNA, indicating double scission | Bos taurus |
| T14C/H44D | significant increase in DNase I activity | Bos taurus |
| T14C/S43A | DNase I activity is similar to that of the wild-type | Bos taurus |
| T14C/S43A/H44D | significant increase in DNase I activity | Bos taurus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Bos taurus |  |
| Mg2+ | in the presence of Mg2+ only, the wild-type cleaves the Mg2+-DNA substrate in a single nicking mode with the formation of only the relaxed open-circular DNA | Bos taurus | |
| Mn2+ | in the presence of Mn2+, the wild-type is able to hydrolyze the Mn2+-substrate forming-linear duplex DNA in addition to the relaxed open-circular DNA, indicating double scission | Bos taurus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| calf thymus DNA + H2O | - |
Bos taurus | 5'-phosphooligonucleotides + ? | - |
? | |
| plasmid DNA + H2O | - |
Bos taurus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNase I | - |
Bos taurus |
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Release 2023.1 (January 2023)
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