Literature summary for 3.1.21.1 extracted from

Mart�nez Valle, F.; Balada, E.; Ordi-Ros, J.; Vilardell-Tarres, M.
DNase 1 and systemic lupus erythematosus (2008), Autoimmun. Rev., 7, 359-363.

Search for more literature for 3.1.21.1

Application

Application	Comment	Organism
medicine	DNase 1 activity is important to prevent immune stimulation and therefore its reduction or loss may result in a high risk to produce ANAs thus contributing to a potential prerequisite to develop a systemic lupus erythematosus-like disease. Survival of DNase 1-treated mice is longer that non-treated mice	Mus musculus
medicine	extracellular DNase 1 is implicated in the chromatin breakdown of necrotic cells in vitro. Connection between DNase 1 activity and the development of human systemic lupus erythematosus. Systemic lupus erythematosus patients have lower DNase 1 activity than patients with rheumatoid arthritis and scleroderma. Usage of DNase 1 as a therapeutic agent in systemic lupus erythematosus	Homo sapiens
medicine	usage of DNase 1 as a therapeutic agent in systemic lupus erythematosus	Bos taurus

Cloned(Commentary)

Cloned (Comment)	Organism
-	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
at 2 A resolution	Bos taurus

Protein Variants

Protein Variants	Comment	Organism
additional information	an A to G transversion in exon 2 at position 172 of the coding sequence in two female systemic lupus erythematosus patients. The mutations result in a replacement of lysine (AAG) with a stop signal (TAG) at residue 5, which results in an inactivated DNAse 1. The two patients have substantially lower levels of DNase 1 activity in the sera than other systemic lupus erythematosus patients without the DNase 1 mutation or healthy controls. Titer of IgG against nucleosomal antigens is 7-8 times higher in patients with the DNase 1 mutation than in other systemic lupus erythematosus patients and 70-80 times higher in the patients with the mutation than in normal controls	Homo sapiens
additional information	young DNase+/- and DNase-/- mice are healthy, but at the age of 6-8 months several animals show symptoms of disease and die. DNase+/- and DNase-/- animals reveal the presence of ANAs and signs of glomerulonephritis, suggesting a correlation between the activity of DNase1 and a systemic lupus erythematosus-like disease. The prevalence of ANAs and glomerulonephritis is higher in female mice than in males and higher in DNase-/- animals than in DNase+/- mice. Female DNase 1-deficient mice contain antibodies against dsDNA	Mus musculus
Q244R	polymorphism of DNase 1, is associated with systemic lupus erythematosus susceptibility but is not correlated with DNase 1 activity	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
G-actin	heat labile inhibitor of DNase 1, released from white blood cells and platelets. Binds to and almost completely inhibits the nucleolytic activity of DNase 1. Inhibition of DNase 1 by actin (about 95% inhibition at equimolar ratio) requires ATP and leads both to the inhibition of DNase 1 and the depolymerization of the actin	Homo sapiens
Zn2+	-	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	two Ca2+ ions that stabilize surface loops as well as an additional metal ion binding site at the active site	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-
Homo sapiens	-	-	-
Mus musculus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
pancreas	-	Bos taurus	-
serum	lupus-prone NZB/NZW mice have significantly lower serum concentrations of DNase 1 than normal mice, and this reduction is not related to the presence of actin	Mus musculus	-
serum	systemic lupus erythematosus patients have lower serum DNase 1 activity compared with healthy controls	Homo sapiens	-
urine	10 phenotypes	Homo sapiens	-
urine	lupus-prone NZB/NZW mice have significantly lower urine concentrations of DNase 1 than normal mice, and this reduction is not related to the presence of actin	Mus musculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
chromatin + H2O	-	Bos taurus	?	-	?
double-stranded DNA + H2O	-	Homo sapiens	5'-phosphooligonucleotides + ?	-	?
double-stranded DNA + H2O	-	Bos taurus	5'-phosphooligonucleotides + ?	-	?

Synonyms

Synonyms	Comment	Organism
DNase 1	-	Mus musculus
DNase 1	-	Homo sapiens
Dnase1	-	Bos taurus

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Release 2023.1 (January 2023)