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Literature summary for 3.1.21.1 extracted from
- Extracellular nuclease from a thermophile, Streptomyces thermonitrificans: production, purification and partial characterization of - double strand preferential - deoxyribonuclease activity (2004), Process Biochem., 40, 1271-1278.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| stable at high concentrations of urea and organic solvents but susceptible to low concentrations of SDS and guanidine hydrochloride | Streptomyces thermovulgaris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Streptomyces thermovulgaris |  |
| Co2+ | - |
Streptomyces thermovulgaris | |
| Cu2+ | - |
Streptomyces thermovulgaris | |
| dithiothreitol | - |
Streptomyces thermovulgaris | |
| EDTA | - |
Streptomyces thermovulgaris | |
| Hg2+ | - |
Streptomyces thermovulgaris | |
| HPO42- | - |
Streptomyces thermovulgaris | |
| Mg2+ | - |
Streptomyces thermovulgaris | |
| NaCl | - |
Streptomyces thermovulgaris | |
| P2O74- | - |
Streptomyces thermovulgaris | |
| Zn2+ | - |
Streptomyces thermovulgaris | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Streptomyces thermovulgaris | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | required | Streptomyces thermovulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22400 | - |
gel filtration | Streptomyces thermovulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces thermovulgaris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Streptomyces thermovulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Streptomyces thermovulgaris | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | - |
Streptomyces thermovulgaris | 5'-phosphooligonucleotides + ? | - |
? | |
| RNA + H2O | - |
Streptomyces thermovulgaris | 5'-phosphooligonucleotides + ? | - |
? | |
| single-stranded DNA + H2O | - |
Streptomyces thermovulgaris | 5'-phosphooligonucleotides + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| extracellular nuclease | - |
Streptomyces thermovulgaris |
| nuclease Stn beta | - |
Streptomyces thermovulgaris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Streptomyces thermovulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Streptomyces thermovulgaris |
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Release 2023.1 (January 2023)
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