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Literature summary for 3.1.21.1 extracted from

  • Chen, C.Y.; Lu, S.C.; Liao, T.H.
    The distinctive functions of the two structural calcium atoms in bovine pancreatic deoxyribonuclease (2002), Protein Sci., 11, 659-668.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli Bos taurus

Protein Variants

Protein Variants Comment Organism
D201A site-directed mutagenesis, exchange of one of 2 Ca2+-binding site residues, 2-3fold increased Km and decrased Vmax compared to the wild-type enzyme, no double-scission ability, no protection by Ca2+ against trypsin inactivation Bos taurus
D99A site-directed mutagenesis, exchange of one of 2 Ca2+-binding site residues, 2-3fold increased Km and unaltered Vmax compared to the wild-type enzyme Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ slight inhibition of mutant D201A Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on, D201 and D99 are involved in binding, conformation, protects wild-type and mutant D99A enzymes against trypsin inactivation, not mutant D201A Bos taurus
Mg2+ dependent on Bos taurus
Mn2+ activates Bos taurus
additional information metal binding and influence on activity, wild-type and mutant enzymes, overview Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from Escherichia coli Bos taurus

Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products preference for double-stranded DNA Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dsDNA + H2O wild-type enzyme and mutant D99A perform double scission on duplex DNA in presence of Mg2+ and Ca2+, not mutant D201A Bos taurus 5'-phosphodinucleotide + 5'-phosphooligonucleotide
-
 ?

Synonyms

Synonyms Comment Organism
pancreatic deoxyribonuclease
-
 Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Bos taurus