Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequences determination and analysis, expression in Escherichia coli BL21(DE3) in the insoluble fraction | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | slight inhibition | Gallus gallus | |
iodoacetate | strong inhibition in presence of Cu2+ | Gallus gallus | |
additional information | resistant to trypsin inactivation in absence of Ca2+ | Gallus gallus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | half-maximal activation at 4 mM | Gallus gallus | |
Mn2+ | half-maximal activation at 1 mM | Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDS-PAGE, x * 30859, isozyme A, mass spectrometry, x * 30882, isozyme B, mass spectrometry | Gallus gallus |
30859 | - |
x * 30000, SDS-PAGE, x * 30859, isozyme A, mass spectrometry, x * 30882, isozyme B, mass spectrometry | Gallus gallus |
30882 | - |
x * 30000, SDS-PAGE, x * 30859, isozyme A, mass spectrometry, x * 30882, isozyme B, mass spectrometry | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | Q9YGI5 | 2 isozymes A and B | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | binds to concanavalin A, contains an N-glycosylation site | Gallus gallus |
Purification (Comment) | Organism |
---|---|
2479fold from pancreas, isozymes A and B to homogeneity | Gallus gallus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products | preference for double-stranded DNA | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
purified isozymes A and B | Gallus gallus |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE, x * 30859, isozyme A, mass spectrometry, x * 30882, isozyme B, mass spectrometry | Gallus gallus |
Synonyms | Comment | Organism |
---|---|---|
DNase | - |
Gallus gallus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Gallus gallus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
inactivation above | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | depending on the metal ion used | Gallus gallus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Gallus gallus | isozyme B, isoelectric focusing | - |
4.43 |
Gallus gallus | isozyme A, isoelectric focusing | - |
4.56 |