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Literature summary for 3.1.21.1 extracted from

  • Yasuda, T.; Takeshita, H.; Iida, R.; Ueki, M.; Nakajima, T.; Kaneko, Y.; Mogi, K.; Kominato, Y.; Kishi, K.
    A single amino acid substitution can shift the optimum pH of DNase I for enzyme activity: biochemical and molecular analysis of the piscine DNase I family (2004), Biochim. Biophys. Acta, 1672, 174-183.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in COS-7 cells Cyprinus carpio
DNA and amino acid sequence determination and analysis, expression in COS-7 cells Pagrus major
DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in COS-7 cells Anguilla japonica

Protein Variants

Protein Variants Comment Organism
D44H shifts the pH optimum from pH 8.0 to lower values of pH 6.5-7.0, 70% reduced activity compared to thw wild-type enzyme Anguilla japonica
E190S pH profile remains similar to the wild-type enzyme with an optimum around pH 8.0 Anguilla japonica
M118R pH profile remains similar to the wild-type enzyme with an optimum around pH 8.0 Anguilla japonica
M236Q pH profile remains similar to the wild-type enzyme with an optimum around pH 8.0 Anguilla japonica
Q134L pH profile remains similar to the wild-type enzyme with an optimum around pH 8.0 Anguilla japonica

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Cyprinus carpio
Ca2+ activates Anguilla japonica
Ca2+ activates Pagrus major
Ca2+ activates Oreochromis mossambicus
Ca2+ activates Takifugu rubripes
Mg2+ activates Cyprinus carpio
Mg2+ activates Anguilla japonica
Mg2+ activates Pagrus major
Mg2+ activates Oreochromis mossambicus
Mg2+ activates Takifugu rubripes

Organism

Organism UniProt Comment Textmining
Anguilla japonica Q5KTT1 eel
-
Cyprinus carpio Q8JIP7 carp
-
Oreochromis mossambicus O42446 tilapia
-
Pagrus major Q5KTT0 sea bream
-
Takifugu rubripes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
10000fold from hepatopancreas Pagrus major
10000fold from pancreas Anguilla japonica
14000fold from hepatopancreas Oreochromis mossambicus
17000fold from hepatopancreas Cyprinus carpio

Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products Asp44 is involved in catalysis, while Met118, Gln134, Glu190, and Met236 are not Anguilla japonica
endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products preference for double-stranded DNA Cyprinus carpio
endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products preference for double-stranded DNA Pagrus major
endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products preference for double-stranded DNA Oreochromis mossambicus
endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products preference for double-stranded DNA Takifugu rubripes

Source Tissue

Source Tissue Comment Organism Textmining
hepatopancreas
-
Cyprinus carpio
-
hepatopancreas
-
Pagrus major
-
hepatopancreas
-
Oreochromis mossambicus
-
pancreas
-
Anguilla japonica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
purified enzyme Cyprinus carpio
additional information
-
purified enzyme Pagrus major
additional information
-
purified enzyme Oreochromis mossambicus

Synonyms

Synonyms Comment Organism
DNase I
-
Cyprinus carpio
DNase I
-
Anguilla japonica
DNase I
-
Pagrus major
DNase I
-
Oreochromis mossambicus
DNase I
-
Takifugu rubripes
More enzyme belongs to the piscine DNase I family Cyprinus carpio
More enzyme belongs to the piscine DNase I family Anguilla japonica
More enzyme belongs to the piscine DNase I family Pagrus major
More enzyme belongs to the piscine DNase I family Oreochromis mossambicus
More enzyme belongs to the piscine DNase I family Takifugu rubripes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Cyprinus carpio
37
-
assay at Anguilla japonica
37
-
assay at Pagrus major
37
-
assay at Oreochromis mossambicus
37
-
assay at Takifugu rubripes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8 Asp44 is responsible for the higher pH optimum compared to vertebrates, residue 44 might have been involved in the evolutionary change of pH optimum for activity from piscine, reptile and amphibia to vertebrates, who possess a His44 residue and a lower pH optimum Pagrus major
8
-
about, Asp44 is responsible for the higher pH optimum compared to vertebrates, residue 44 might have been involved in the evolutionary change of pH optimum for activity from piscine, reptile and amphibia to vertebrates, who possess a His44 residue and a lower pH optimum Cyprinus carpio
8
-
about, Asp44 is responsible for the higher pH optimum compared to vertebrates, residue 44 might have been involved in the evolutionary change of pH optimum for activity from piscine, reptile and amphibia to vertebrates, who possess a His44 residue and a lower pH optimum Anguilla japonica
8
-
about, Asp44 is responsible for the higher pH optimum compared to vertebrates, residue 44 might have been involved in the evolutionary change of pH optimum for activity from piscine, reptile and amphibia to vertebrates, who possess a His44 residue and a lower pH optimum Oreochromis mossambicus
8
-
about, Asp44 is responsible for the higher pH optimum compared to vertebrates, residue 44 might have been involved in the evolutionary change of pH optimum for activity from piscine, reptile and amphibia to vertebrates, who possess a His44 residue and a lower pH optimum Takifugu rubripes