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Literature summary for 3.1.21.1 extracted from
- Deoxyribonuclease I (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 4, 289-311.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| Ca2+ is able to protect DNase completely against inactivation by trypsin | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | inactivation, reversal by the addition of 4 mM CaCl2, no inactivation if CaCl2 is present during the reducing reaction | Bos taurus |  |
| calf spleen inhibitor protein II | molecular weight: 59000 Da, forms an inhibitory uni-uni molecular complex with DNase I, maximum stability at pH 7 | Bos taurus | |
| calf thymus inhibitor protein | molecular weight: 49000 Da, maximum stability at pH 6 | Bos taurus | |
| iodoacetate | formation of a 3-carboxymethyl histidine per molecule, in the presence of 0.1 M Mn2+ gradual inactivation | Bos taurus | |
| NaCl | - |
Bos taurus | |
| oligonucleotides | competitive inhibition | Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | no lag phase in the reaction obtained, if only Ca2+ is present or both Mg2+ and Ca2+ are present, double strand digestion preferred | Bos taurus | |
| Co2+ | reaction performs mainly double strand scissions, Co2+ is a better activator than other divalent metals | Bos taurus | |
| Mg2+ | only single strand cleavage occurs during the lag period of the reaction if only Mg2+ is present | Bos taurus | |
| Mn2+ | reaction performs mainly double strand scissions | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
amino acid analysis | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | Bos taurus | preference for double-stranded DNA | 5'-phosphooligonucleotides + ? | differences in the composition of digests obtained in the presence of Mn2+ or Mg2+, differences between the early and terminal stages of the reaction, with DNA as substrate early cleavages are directed towards the center of the molecule and are predominantly single-strand nicks, in the latter part of the reaction the purine-p-pyrimidine bond is preferentially cleaved | ? | |
| double-stranded DNA + H2O | Bos taurus | major function is to inflict nicks during early stages of hydrolytic attack on DNA, participation in repair phenomena | 5'-phosphooligonucleotides + ? | differences in the composition of digests obtained in the presence of Mn2+ or Mg2+, differences between the early and terminal stages of the reaction, with DNA as substrate early cleavages are directed towards the center of the molecule and are predominantly single-strand nicks, in the latter part of the reaction the purine-p-pyrimidine bond is preferentially cleaved | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
4 active compounds: A, B, C, D | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | DNase A: 2 N-acetylglucosamine residues, 6 mannose residues, DNase B: 1 sialic acid residue, 3 N-acetylglucosamine residues, 5 mannose residues | Bos taurus |
| glycoprotein | DNase A with a neutral carbohydrate side-chain, DNase B with a sialic acid carbohydrate side-chain | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| d-ApApTp + H2O | - |
Bos taurus | pTp + d-ApA | - |
? | |
| DNA + H2O | preference for native DNA | Bos taurus | 5'-phosphooligonucleotides + ? | produces nicks on one strand in preference to scission of both strands, autoretardation causes the continuous formation of products which are poorer substrates than those from which they are derived | ? | |
| double-stranded DNA + H2O | preference for double-stranded DNA | Bos taurus | 5'-phosphooligonucleotides + ? | differences in the composition of digests obtained in the presence of Mn2+ or Mg2+, differences between the early and terminal stages of the reaction, with DNA as substrate early cleavages are directed towards the center of the molecule and are predominantly single-strand nicks, in the latter part of the reaction the purine-p-pyrimidine bond is preferentially cleaved | ? | |
| double-stranded DNA + H2O | major function is to inflict nicks during early stages of hydrolytic attack on DNA, participation in repair phenomena | Bos taurus | 5'-phosphooligonucleotides + ? | differences in the composition of digests obtained in the presence of Mn2+ or Mg2+, differences between the early and terminal stages of the reaction, with DNA as substrate early cleavages are directed towards the center of the molecule and are predominantly single-strand nicks, in the latter part of the reaction the purine-p-pyrimidine bond is preferentially cleaved | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNase A | - |
Bos taurus |
| DNase B | - |
Bos taurus |
| DNase C | - |
Bos taurus |
| DNase D | - |
Bos taurus |
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