Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | inhibition after treatment with EGTA | Homo sapiens | |
EDTA | 50% inhibition at 0.3 mM | Homo sapiens | |
NaCl | 50% inhibition at 80 mM and pH 5.8 and at 165 mM and pH 7.0 | Homo sapiens | |
Zn2+ | complete inhibition at 1 mM | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation synergistic with Mg2+ | Homo sapiens | |
Mg2+ | activation synergistic with Ca2+ | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | 38000 | for serum and pancreas DNase I, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | Homo sapiens | - |
5'-phosphooligonucleotides + ? | double-strand breaks occure more rapidly in the presence of Ca2+ | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity by chromatography techniques for serum and pancreas DNase I | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Homo sapiens | - |
serum | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Homo sapiens | 5'-phosphooligonucleotides + ? | - |
? | |
double-stranded DNA + H2O | - |
Homo sapiens | 5'-phosphooligonucleotides + ? | double-strand breaks occure more rapidly in the presence of Ca2+ | ? |
Synonyms | Comment | Organism |
---|---|---|
EndoDNase | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
in the presence of Mg-EGTA | Homo sapiens |
7 | - |
serum DNase I in the presence of Mg2+ and Ca2+ | Homo sapiens |
7.5 | - |
for pancreas DNase I in the presence of Mg2+ and Ca2+ | Homo sapiens |