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Literature summary for 3.1.21.1 extracted from

  • Love, J.D.; Hewitt, R.R.
    The realtionship between human serum and human pancreatic DNase I (1979), J. Biol. Chem., 254, 12588-12594.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol inhibition after treatment with EGTA Homo sapiens
EDTA 50% inhibition at 0.3 mM Homo sapiens
NaCl 50% inhibition at 80 mM and pH 5.8 and at 165 mM and pH 7.0 Homo sapiens
Zn2+ complete inhibition at 1 mM Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation synergistic with Mg2+ Homo sapiens
Mg2+ activation synergistic with Ca2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000 38000 for serum and pancreas DNase I, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded DNA + H2O Homo sapiens
-
5'-phosphooligonucleotides + ? double-strand breaks occure more rapidly in the presence of Ca2+ ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by chromatography techniques for serum and pancreas DNase I Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
Homo sapiens
-
serum
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
Homo sapiens 5'-phosphooligonucleotides + ?
-
?
double-stranded DNA + H2O
-
Homo sapiens 5'-phosphooligonucleotides + ? double-strand breaks occure more rapidly in the presence of Ca2+ ?

Synonyms

Synonyms Comment Organism
EndoDNase
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
in the presence of Mg-EGTA Homo sapiens
7
-
serum DNase I in the presence of Mg2+ and Ca2+ Homo sapiens
7.5
-
for pancreas DNase I in the presence of Mg2+ and Ca2+ Homo sapiens