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Literature summary for 3.1.21.1 extracted from
- Purification and enzymatic characterization of three EndoDNase isoenzymes from Physarum polycephalum (1980), J. Biochem., 87, 651-661.
General Stability
| General Stability | Organism |
|---|---|
| less active in phosphate buffer | Physarum polycephalum |
| sensitives to repeated freezing and thawing | Physarum polycephalum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | complete inhibition at 1-10 mM | Physarum polycephalum |  |
| Cu2+ | complete inhibition at 1-10 mM | Physarum polycephalum | |
| EDTA | - |
Physarum polycephalum | |
| EGTA | activity inhibited | Physarum polycephalum | |
| Zn2+ | complete inhibition at 1-10 mM | Physarum polycephalum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 2.0-7.5 mM for DNases A, B or C | Physarum polycephalum | |
| Mn2+ | 0.8-2.5 mM for DNases A, B or C | Physarum polycephalum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16000 | 17000 | gel filtration, SDS-PAGE | Physarum polycephalum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | Physarum polycephalum | preference for double-stranded DNA | 5'-phosphooligonucleotides + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Physarum polycephalum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity by chromatography techniques, isoelectric focusing, gel filtration | Physarum polycephalum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| plasmodium | microplasmodium | Physarum polycephalum | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 10 mM Tris-HCl, pH 7.0, 10 mM KCl, 5 M urea, 6 months | Physarum polycephalum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | - |
Physarum polycephalum | 5'-phosphooligonucleotides + ? | - |
? | |
| double-stranded DNA + H2O | preference for double-stranded DNA | Physarum polycephalum | 5'-phosphooligonucleotides + ? | - |
? | |
| heat-denatured DNA + H2O | - |
Physarum polycephalum | 5'-phosphooligonucleotides + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 16000-17000, four active fractions | Physarum polycephalum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNase A | - |
Physarum polycephalum |
| DNase B | - |
Physarum polycephalum |
| DNase C | - |
Physarum polycephalum |
| EndoDNase | - |
Physarum polycephalum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.25 | - |
for DNase A | Physarum polycephalum |
| 8.5 | - |
for DNase B | Physarum polycephalum |
| 8.75 | - |
for DNase C | Physarum polycephalum |
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