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Literature summary for 3.1.21.1 extracted from

  • Moore, S.
    Pancreatic DNase (1981), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 14, 281-296.
No PubMed abstract available

Application

Application Comment Organism
analysis study of chromatin structure, visualizing cellular microfilaments by DNase I-actin interaction Bos taurus
medicine inhibition of viral synthesis by DNase I in cell cultures Bos taurus

General Stability

General Stability Organism
extremely sensitive to inactivation by proteases in the absence of Ca2+ Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
2-Nitro-5-thiocyanobenzoic acid inactivation by cleavage of peptide chain at positions 14, 40, 72 and 135 Bos taurus
Ca2+ inhibitory above 1 mM Bos taurus
carbodiimide presence of divalent cations slows the rate of inactivation Bos taurus
EGTA inhibition at 0.01 mM in the presence of 2.5 mM Mg2+ Bos taurus
G-actin DNase I causes depolymerization of F-actin to form a stable complex of 1 mol DNase I with 1 mol G-actin, this complex inhibits DNase I activity Bos taurus
iodoacetate inhibition in the presence of Mn2+ or Ca2+ at pH 7.2 Bos taurus
methanesulfonylchloride inactivation at pH 5.0 Bos taurus
N-bromosuccinimide inactivation by modification at Trp155 Bos taurus
NaCl Na-DNA is inhibitory Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ can substitute for Ca2+, but less effective Bos taurus
Ca2+ resistance to proteolysis conveyed by Ca2+ Bos taurus
Ca2+ activation synergistic with Mg2+ Bos taurus
Co2+ can substitute for Mg2+, but only 10% efficiency Bos taurus
Mg2+ enzyme about 99.5% inactive if Ca2+ contamination is reduced to a minimum, must bind to the substrate, whereas Ca2+ must bind to the enzyme Bos taurus
Mn2+ can substitute for Mg2+ Bos taurus
Sr2+ can substitute for Ca2+, but less effective Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30070
-
calculated from amino acid and carbohydrate composition for DNase A Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded DNA + H2O Bos taurus double-strand scission obtained if affinity for the substrate is at a maximum in the presence of both Ca2+ and Mg2+, single-strand scission and changes in specificity are associated with suboptimal concentrations of Ca2+ 5'-phosphooligonucleotides + ? in the presence of divalent cations that give less maximum activity consistent yields of long oligonucleotides lacking dA at the 3'-end ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
4 active compounds: A, B, C, D
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein DNase D contains sialic acid in the carbohydrate portion Bos taurus
glycoprotein carbohydrate-chain at Asn 18 Bos taurus
glycoprotein DNase A with a neutral carbohydrate side-chain, DNase B with a sialic acid carbohydrate side-chain Bos taurus

Purification (Commentary)

Purification (Comment) Organism
conconavalin A-agarose as an effective step for DNases with neutral carbohydrate side-chains, preparing a protease free DNase I Bos taurus

Storage Stability

Storage Stability Organism
37°C, protease free preparation, absence of Ca2+, pH 8.0, more than 10 days Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
crab d(A-T) polymer + H2O unique polymer of alterating A and T contains about 3% G and C residues integrated into its structure Bos taurus 5'-hexanucleotides + ? enriched in C and G, sugar specificity may be limited to the nucleotide following the point of cleavage ?
double-stranded DNA + H2O double-strand scission obtained if affinity for the substrate is at a maximum in the presence of both Ca2+ and Mg2+, single-strand scission and changes in specificity are associated with suboptimal concentrations of Ca2+ Bos taurus 5'-phosphooligonucleotides + ? in the presence of divalent cations that give less maximum activity consistent yields of long oligonucleotides lacking dA at the 3'-end ?
NO2-Ph-pdTp-NO2Ph + H2O rapidly hydrolyzed at a single bond Bos taurus p-nitrophenol + NO2-Ph-pdT-3'-phosphate
-
?

Synonyms

Synonyms Comment Organism
DNase A
-
Bos taurus
DNase B
-
Bos taurus
DNase C
-
Bos taurus
DNase D
-
Bos taurus