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Literature summary for 3.1.21.1 extracted from

  • Nagae, S.; Nakayama, J.; Nakano, I.; Anai, M.
    Purification and properties of a neutral endodeoxyribonuclease from rat small intestinal mucosa (1982), Biochemistry, 21, 1339-1344.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin 0.01 mg/reaction volume stimulates about 50% at both pH 6.2 and 7.7 Rattus sp.
N-ethylmaleimide stimulation about 10-20% at both pH 6.2 and 7.7 Rattus sp.

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol inhibition, but reversal by addition of 3 mM CaCl2 Rattus sp.
Cu2+ complete inhibition Rattus sp.
EDTA
-
Rattus sp.
G-actin
-
Rattus sp.
iodoacetate complete inhibition Rattus sp.
NaCl
-
Rattus sp.
Zn2+ complete inhibition Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Rattus sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Rattus sp.
Mg2+ activation synergistic with Ca2+ Rattus sp.
Mn2+
-
Rattus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
gel filtration, sucrose density gradient centrifugation Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded DNA + H2O Rattus sp. introduces preferentially breaks on the 5' side of dT 5'-phosphooligonucleotides + ?
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
Ethylene glycol 80% loss of activity during overnight at 0°C Rattus sp.

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by affinity chromatography Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
intestinal mucosa
-
Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.2
-
-
Rattus sp.

Storage Stability

Storage Stability Organism
-20°C, 20 mM Tris-HCl, pH 7.5, 30% glycerol, 6 months Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O degrades native DNA 1.5times faster than denatured DNA Rattus sp. 5'-phosphooligonucleotides + ? half the 5'-termini are deoxythymidinemonophosphate, 90% of the products are larger than pentanucleotides ?
double-stranded DNA + H2O introduces preferentially breaks on the 5' side of dT Rattus sp. 5'-phosphooligonucleotides + ?
-
?
heat-denatured DNA + H2O
-
Rattus sp. 5'-phosphooligonucleotides + ? 90% of the products are larger than pentanucleotides ?
relaxed circular plasmid DNA + H2O acts more rapidly on circular duplex DNA than on supercoiled DNA Rattus sp. 5'-phosphooligonucleotides + ?
-
?
supercoiled plasmid DNA + H2O
-
Rattus sp. linear DNA + ? superhelical form converted to circular-relaxed and linear forms ?

Subunits

Subunits Comment Organism
monomer
-
Rattus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-30
-
no loss of activity in crude extract Rattus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
in 25 mM cacodylate hydrochlorid and 3 mM Co2+ Rattus sp.
7.7
-
in 25 mM Tris-HCl and 1 mM Mn2+ Rattus sp.