Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | 0.01 mg/reaction volume stimulates about 50% at both pH 6.2 and 7.7 | Rattus sp. | |
N-ethylmaleimide | stimulation about 10-20% at both pH 6.2 and 7.7 | Rattus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | inhibition, but reversal by addition of 3 mM CaCl2 | Rattus sp. | |
Cu2+ | complete inhibition | Rattus sp. | |
EDTA | - |
Rattus sp. | |
G-actin | - |
Rattus sp. | |
iodoacetate | complete inhibition | Rattus sp. | |
NaCl | - |
Rattus sp. | |
Zn2+ | complete inhibition | Rattus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Rattus sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Rattus sp. | |
Mg2+ | activation synergistic with Ca2+ | Rattus sp. | |
Mn2+ | - |
Rattus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
gel filtration, sucrose density gradient centrifugation | Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | Rattus sp. | introduces preferentially breaks on the 5' side of dT | 5'-phosphooligonucleotides + ? | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Ethylene glycol | 80% loss of activity during overnight at 0°C | Rattus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity by affinity chromatography | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestinal mucosa | - |
Rattus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.2 | - |
- |
Rattus sp. |
Storage Stability | Organism |
---|---|
-20°C, 20 mM Tris-HCl, pH 7.5, 30% glycerol, 6 months | Rattus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | degrades native DNA 1.5times faster than denatured DNA | Rattus sp. | 5'-phosphooligonucleotides + ? | half the 5'-termini are deoxythymidinemonophosphate, 90% of the products are larger than pentanucleotides | ? | |
double-stranded DNA + H2O | introduces preferentially breaks on the 5' side of dT | Rattus sp. | 5'-phosphooligonucleotides + ? | - |
? | |
heat-denatured DNA + H2O | - |
Rattus sp. | 5'-phosphooligonucleotides + ? | 90% of the products are larger than pentanucleotides | ? | |
relaxed circular plasmid DNA + H2O | acts more rapidly on circular duplex DNA than on supercoiled DNA | Rattus sp. | 5'-phosphooligonucleotides + ? | - |
? | |
supercoiled plasmid DNA + H2O | - |
Rattus sp. | linear DNA + ? | superhelical form converted to circular-relaxed and linear forms | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Rattus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
-30 | - |
no loss of activity in crude extract | Rattus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
in 25 mM cacodylate hydrochlorid and 3 mM Co2+ | Rattus sp. |
7.7 | - |
in 25 mM Tris-HCl and 1 mM Mn2+ | Rattus sp. |