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Literature summary for 3.1.21.1 extracted from
- Human urine DNase I: Immunological identity with human pancreatic DNase I, and enzyme and proteochemical properties of the enzyme (1984), J. Biochem., 95, 1399-1406.
General Stability
| General Stability | Organism |
|---|---|
| less active in phosphate buffer | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | secreted into urine | Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Homo sapiens |  |
| Co2+ | - |
Homo sapiens | |
| Mg2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
SDS-PAGE, gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | Homo sapiens | - |
5'-phosphooligonucleotides + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity by 6step chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| urine | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
units defined as absorbance at 260 nm/ml assay solution | Homo sapiens |
| additional information | - |
1683 units/mg protein | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | degrades native DNA 2times faster than denatured DNA | Homo sapiens | 5'-phosphooligonucleotides + ? | - |
? | |
| double-stranded DNA + H2O | - |
Homo sapiens | 5'-phosphooligonucleotides + ? | - |
? | |
| heat-denatured DNA + H2O | - |
Homo sapiens | 5'-phosphooligonucleotides + ? | - |
? | |
| poly(dA) + H2O | - |
Homo sapiens | 5'-phosphooligonucleotides + ? | - |
? | |
| poly(dT) + H2O | - |
Homo sapiens | 5'-phosphooligonucleotides + ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
stable up to at pH 6.5, loss of activity at 60°C | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | stable within the range for 24 h at 4°C | Homo sapiens |
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