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Literature summary for 3.1.21.1 extracted from
- Purification and characterization of an endonuclease from fruiting caps of basidiomycete Coprinuns cinereus (1988), Eur. J. Biochem., 174, 725-732.
General Stability
| General Stability | Organism |
|---|---|
| sensitives to repeated freezing and thawing | Coprinopsis cinerea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EGTA | activity inhibited | Coprinopsis cinerea |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | in presence of Ca2+ less sensitive to buffer strength | Coprinopsis cinerea | |
| Mg2+ | absolute requirement for activity, highest activity if both Mg2+ and Ca2+ are present | Coprinopsis cinerea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12000 | - |
SDS-PAGE | Coprinopsis cinerea |
| 14000 | - |
SDS-PAGE | Coprinopsis cinerea |
| 22000 | - |
gel filtration | Coprinopsis cinerea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | Coprinopsis cinerea | preference for single-stranded and supercoiled DNA | 5'-phosphooligonucleotides + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Coprinopsis cinerea | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| basidiocarp | - |
Coprinopsis cinerea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
21 units/min/ng DNA digested | Coprinopsis cinerea |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 10 mM Tris/HCl, pH 7.4, 50% v/v glycerol, 2 years | Coprinopsis cinerea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | preference for single-stranded and supercoiled DNA | Coprinopsis cinerea | 5'-phosphooligonucleotides + ? | - |
? | |
| relaxed circular plasmid DNA + H2O | - |
Coprinopsis cinerea | 5'-phosphooligonucleotides + ? | - |
? | |
| single-stranded circular DNA + H2O | preference for single-stranded regions | Coprinopsis cinerea | 5'-phosphooligonucleotides + ? | - |
? | |
| supercoiled plasmid DNA + H2O | - |
Coprinopsis cinerea | linear DNA + ? | formation of a relaxed circle that subsequently converts to linear DNA if it is nicked on the other strand at the same position | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 12000 and 1 * 14000, gel filtration, SDS-PAGE | Coprinopsis cinerea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Coprinopsis cinerea |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 60 | activity remains unchanged | Coprinopsis cinerea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable for 20 min | Coprinopsis cinerea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Coprinopsis cinerea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | 64% activity below pH 5.0, 80% activity above pH 8.0 | Coprinopsis cinerea |
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