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Literature summary for 3.1.21.1 extracted from
- Purification and characterization of tilapia (Oreochromis mossambicus) deoxyribonuclease I. Primary structure and cDNA sequence (1997), Eur. J. Biochem., 249, 786-791.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| deducing amino acid composition from cDNA | Oreochromis mossambicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetate | 50% inhibition at 0.1 M in presence of 4 mM CuCl2 after 15 min | Oreochromis mossambicus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | highest activity at 10 mM, doubled activity upon addition of 1 mM Ca2+ | Oreochromis mossambicus | |
| Mn2+ | highest activity at 1 mM, addition of Ca2+ doesn't change activity | Oreochromis mossambicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
gel filtration, SDS-PAGE, thin-layer IEF, MS analysis | Oreochromis mossambicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | Oreochromis mossambicus | - |
5'-phosphooligonucleotides + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oreochromis mossambicus | - |
tilapia fish | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | carbohydrate-chain at Asn 18 | Oreochromis mossambicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity by 4step chromatography | Oreochromis mossambicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatopancreas | - |
Oreochromis mossambicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
units defined as absorbance at 260 nm/ml assay solution | Oreochromis mossambicus |
| additional information | - |
670 units/mg protein | Oreochromis mossambicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | - |
Oreochromis mossambicus | 5'-phosphooligonucleotides + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 30000, SDS-PAGE, gel filtration | Oreochromis mossambicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
inactivation after 10 min incubation | Oreochromis mossambicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
in the presence of Mg2+ | Oreochromis mossambicus |
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