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Literature summary for 3.1.2.6 extracted from

  • Ozyamak, E.; Black, S.S.; Walker, C.A.; Maclean, M.J.; Bartlett, W.; Miller, S.; Booth, I.R.
    The critical role of S-lactoylglutathione formation during methylglyoxal detoxification in Escherichia coli (2010), Mol. Microbiol., 78, 1577-1590.
    View publication on PubMedView publication on EuropePMC
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Organism

Organism UniProt Comment Textmining
Escherichia coli
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-
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Synonyms

Synonyms Comment Organism
GloB
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 Escherichia coli
GlxII
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 Escherichia coli

General Information

General Information Comment Organism
physiological function a DELTAgloB mutant is as tolerant of methylglyoxal as the parent, despite having the same degree of inhibition of MG detoxification as a DELTAgloA strain. The DgloB mutant grows at a similar rate to the parent in K0.2 minimal medium and exhibits no obvious growth phenotype. Addition of 0.7 mM MG to both parent andmutant strains, in early exponential phase, causes immediate growth inhibition without recovery over the course of the experiment. Mehtylglyoxal disappeares from the medium in a linear fashion and the rate is greatly reduced in DELTAgloB cultures. Increased expression of GlxII from a multicopyplasmid sensitizes Escherichia coli to methylglyoxal. S-lactoylglutathione pools, KefGB potassium efflux system activity and cytoplasmic pH are linked and very sensitive to changes in the activity of GlxI and GlxII Escherichia coli