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Literature summary for 3.1.2.6 extracted from

  • Campos-Bermudez, V.A.; Moran-Barrio, J.; Costa-Filho, A.J.; Vila, A.J.
    Metal-dependent inhibition of glyoxalase II: A possible mechanism to regulate the enzyme activity (2010), J. Inorg. Biochem., 104, 726-731.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.2.6

Cloned(Commentary)

Cloned (Comment) Organism
pET32-gloB vector overexpressed in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
glutathione metal-selective end product inhibition. Thiol-Fe bond is formed between the Cys moiety of glutathione and the metal site, the dinuclear iron sites show an enhanced antiferromagnetic coupling in the GloB-glutathione adduct Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ behavior of Fe-GloB resembles that of Zn-GloB. Thiol-Fe bond is formed between the Cys moiety of glutathione and the metal site, the dinuclear iron sites show an enhanced antiferromagnetic coupling in the GloB-glutathione adduct. GloB-glutathione complex displays S-thiol-FeIII and S-thiol-FeII: charge transfer bands Salmonella enterica subsp. enterica serovar Typhimurium
Fe3+ GloB-glutathione complex displays S-thiol-FeIII and S-thiol-FeII: charge transfer bands Salmonella enterica subsp. enterica serovar Typhimurium
Mn2+ MnII-thiolate bonds are less covalent and weaker than iron–thiolate bonds Salmonella enterica subsp. enterica serovar Typhimurium
Zn2+ behavior of Zn-GloB resembles that of Fe-GloB Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium 14028s
-
-
-

Purification (Commentary)

Purification (Comment) Organism
iron-enriched GloB, manganese-enriched GloB and zinc-enriched GloB Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-D-lactoylglutathione + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium D-lactate + glutathione
-
 ?
S-D-lactoylglutathione + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium 14028s D-lactate + glutathione
-
 ?

Synonyms

Synonyms Comment Organism
GloB
-
 Salmonella enterica subsp. enterica serovar Typhimurium
GLX2
-
 Salmonella enterica subsp. enterica serovar Typhimurium
glyoxalase II
-
 Salmonella enterica subsp. enterica serovar Typhimurium
hydroxyacylglutathione hydrolase
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
170
-
 S-D-lactoylglutathione in 10 mM MOPS buffer at pH 7.2, at 25 °C Salmonella enterica subsp. enterica serovar Typhimurium

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
 Fe- and Zn-GloB is inhibited at submillimolar concentrations of glutathione, in 10 mM MOPS buffer, 0.2 M NaCl, pH 7.2, at 30 °C Salmonella enterica subsp. enterica serovar Typhimurium additional information
1.5
-
 Mn-GloB, in 10 mM MOPS buffer, 0.2 M NaCl, pH 7.2, at 30 °C Salmonella enterica subsp. enterica serovar Typhimurium glutathione

General Information

General Information Comment Organism
physiological function assembly of a specific metal-enriched form of GLX2 may be a mechanism to regulate the enzyme activity in vivo Salmonella enterica subsp. enterica serovar Typhimurium