Cloned (Comment) | Organism |
---|---|
pET32-gloB vector overexpressed in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutathione | metal-selective end product inhibition. Thiol-Fe bond is formed between the Cys moiety of glutathione and the metal site, the dinuclear iron sites show an enhanced antiferromagnetic coupling in the GloB-glutathione adduct | Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | behavior of Fe-GloB resembles that of Zn-GloB. Thiol-Fe bond is formed between the Cys moiety of glutathione and the metal site, the dinuclear iron sites show an enhanced antiferromagnetic coupling in the GloB-glutathione adduct. GloB-glutathione complex displays S-thiol-FeIII and S-thiol-FeII: charge transfer bands | Salmonella enterica subsp. enterica serovar Typhimurium | |
Fe3+ | GloB-glutathione complex displays S-thiol-FeIII and S-thiol-FeII: charge transfer bands | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mn2+ | MnII-thiolate bonds are less covalent and weaker than ironthiolate bonds | Salmonella enterica subsp. enterica serovar Typhimurium | |
Zn2+ | behavior of Zn-GloB resembles that of Fe-GloB | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Salmonella enterica subsp. enterica serovar Typhimurium 14028s | - |
- |
- |
Purification (Comment) | Organism |
---|---|
iron-enriched GloB, manganese-enriched GloB and zinc-enriched GloB | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-D-lactoylglutathione + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | D-lactate + glutathione | - |
? | |
S-D-lactoylglutathione + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium 14028s | D-lactate + glutathione | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GloB | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
GLX2 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
glyoxalase II | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
hydroxyacylglutathione hydrolase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
170 | - |
S-D-lactoylglutathione | in 10 mM MOPS buffer at pH 7.2, at 25 °C | Salmonella enterica subsp. enterica serovar Typhimurium |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
Fe- and Zn-GloB is inhibited at submillimolar concentrations of glutathione, in 10 mM MOPS buffer, 0.2 M NaCl, pH 7.2, at 30 °C | Salmonella enterica subsp. enterica serovar Typhimurium | additional information | |
1.5 | - |
Mn-GloB, in 10 mM MOPS buffer, 0.2 M NaCl, pH 7.2, at 30 °C | Salmonella enterica subsp. enterica serovar Typhimurium | glutathione |
General Information | Comment | Organism |
---|---|---|
physiological function | assembly of a specific metal-enriched form of GLX2 may be a mechanism to regulate the enzyme activity in vivo | Salmonella enterica subsp. enterica serovar Typhimurium |