BRENDA - Enzyme Database
show all sequences of 3.1.2.4

Purification and partial characterization of 3-hydroxyisobutyryl-coenzyme A hydrolase of rat liver

Shimomura, Y.; Murakami, T.; Fujitsuka, N.; Nakai, N.; Sato, Y.; Sugiyama, S.; Shimomura, N.; Irwin, J.; Hawes, J.W.; Harris, R.A.; J. Biol. Chem. 269, 14248-14253 (1994)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
acetoacetyl-CoA
competitive
Rattus norvegicus
acetyl-CoA
competitive
Rattus norvegicus
DL-3-hydroxybutyryl-CoA
competitive
Rattus norvegicus
DL-methylmalonyl-CoA
competitive
Rattus norvegicus
isobutyryl-CoA
competitive
Rattus norvegicus
malonyl-CoA
competitive
Rattus norvegicus
n-valeryl-CoA
competitive
Rattus norvegicus
propionyl-CoA
competitive
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.006
-
3-hydroxy-2-methylpropanoyl-CoA
-
Rattus norvegicus
0.025
-
3-hydroxypropionyl-CoA
-
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3-hydroxy-2-methylpropanoyl-CoA + H2O
Rattus norvegicus
enzyme is involved in Val catabolism. The enzyme protects cells against toxic effects of methacrylyl-CoA, an intermediate in the Val pathway occuring upstream of 3-hydroxy-2-methylpropanoyl-CoA
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Rattus norvegicus
-
heart
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
muscle
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
427
-
-
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(S)-methylmalonyl-CoA + H2O
-
94558
Rattus norvegicus
CoA + methylmalonate
-
-
-
?
3-hydroxy-2-methylpropanoyl-CoA + H2O
-
94558
Rattus norvegicus
CoA + 3-hydroxy-2-methylpropanoate
-
-
-
?
3-hydroxy-2-methylpropanoyl-CoA + H2O
enzyme is involved in Val catabolism. The enzyme protects cells against toxic effects of methacrylyl-CoA, an intermediate in the Val pathway occuring upstream of 3-hydroxy-2-methylpropanoyl-CoA
94558
Rattus norvegicus
?
-
-
-
?
3-hydroxypropanoyl-CoA + H2O
-
94558
Rattus norvegicus
CoA + 3-hydroxypropanoate
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 36000, SDS-PAGE
Rattus norvegicus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
150
-
3-hydroxy-2-methylpropanoyl-CoA
-
Rattus norvegicus
266
-
3-hydroxy-2-methylpropanoyl-CoA
-
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
hydrolysis of (S)-methylmalonyl-CoA
Rattus norvegicus
8
-
hydrolysis of 3-hydroxy-2-methylpropanoyl-CoA
Rattus norvegicus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
7
pH 5.0, about 30% of maximal activity, pH 7.0: 65% of maximal activity, hydrolysis of (S)-methylmalonyl-CoA
Rattus norvegicus
5
10.5
pH 5.0: about 40% of maximal activity, pH 10.5: about 80% of maximal activity, hydrolysis of 3-hydroxy-2-methylpropanoyl-CoA
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetoacetyl-CoA
competitive
Rattus norvegicus
acetyl-CoA
competitive
Rattus norvegicus
DL-3-hydroxybutyryl-CoA
competitive
Rattus norvegicus
DL-methylmalonyl-CoA
competitive
Rattus norvegicus
isobutyryl-CoA
competitive
Rattus norvegicus
malonyl-CoA
competitive
Rattus norvegicus
n-valeryl-CoA
competitive
Rattus norvegicus
propionyl-CoA
competitive
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.006
-
3-hydroxy-2-methylpropanoyl-CoA
-
Rattus norvegicus
0.025
-
3-hydroxypropionyl-CoA
-
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3-hydroxy-2-methylpropanoyl-CoA + H2O
Rattus norvegicus
enzyme is involved in Val catabolism. The enzyme protects cells against toxic effects of methacrylyl-CoA, an intermediate in the Val pathway occuring upstream of 3-hydroxy-2-methylpropanoyl-CoA
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Rattus norvegicus
-
heart
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
muscle
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
427
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(S)-methylmalonyl-CoA + H2O
-
94558
Rattus norvegicus
CoA + methylmalonate
-
-
-
?
3-hydroxy-2-methylpropanoyl-CoA + H2O
-
94558
Rattus norvegicus
CoA + 3-hydroxy-2-methylpropanoate
-
-
-
?
3-hydroxy-2-methylpropanoyl-CoA + H2O
enzyme is involved in Val catabolism. The enzyme protects cells against toxic effects of methacrylyl-CoA, an intermediate in the Val pathway occuring upstream of 3-hydroxy-2-methylpropanoyl-CoA
94558
Rattus norvegicus
?
-
-
-
?
3-hydroxypropanoyl-CoA + H2O
-
94558
Rattus norvegicus
CoA + 3-hydroxypropanoate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 36000, SDS-PAGE
Rattus norvegicus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
150
-
3-hydroxy-2-methylpropanoyl-CoA
-
Rattus norvegicus
266
-
3-hydroxy-2-methylpropanoyl-CoA
-
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
hydrolysis of (S)-methylmalonyl-CoA
Rattus norvegicus
8
-
hydrolysis of 3-hydroxy-2-methylpropanoyl-CoA
Rattus norvegicus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
7
pH 5.0, about 30% of maximal activity, pH 7.0: 65% of maximal activity, hydrolysis of (S)-methylmalonyl-CoA
Rattus norvegicus
5
10.5
pH 5.0: about 40% of maximal activity, pH 10.5: about 80% of maximal activity, hydrolysis of 3-hydroxy-2-methylpropanoyl-CoA
Rattus norvegicus
Other publictions for EC 3.1.2.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750422
Zamani
A putative 3-hydroxyisobutyry ...
Sinorhizobium meliloti, Sinorhizobium fredii, Sinorhizobium fredii NGR234, Sinorhizobium meliloti RmP798
Environ. Microbiol.
19
218-236
2017
-
-
-
-
-
-
-
-
-
-
-
4
-
17
-
-
-
-
-
-
-
-
4
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
6
6
-
-
-
751805
Pan
3-Hydroxyisobutyryl-CoA hydro ...
Phaeodactylum tricornutum
Philos. Trans. R. Soc. Lond. B Biol. Sci.
372
20160409
2017
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
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2
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-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
710015
Dong
Disruption of Arabidopsis CHY1 ...
Arabidopsis thaliana
Mol. Plant
2
59-72
2009
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
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-
-
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3
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1
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1
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-
-
-
-
-
-
-
2
2
-
-
-
710261
Ibdah
Arabidopsis Chy1 null mutants ...
Arabidopsis thaliana
Plant Biol.
11
574-581
2009
-
-
1
-
-
-
1
1
-
1
-
-
-
7
-
-
1
-
-
1
-
-
4
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
1
-
1
-
-
4
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
684647
Lee
Biosynthesis of enantiopure (S ...
Bacillus cereus
Appl. Microbiol. Biotechnol.
79
633-641
2008
-
-
1
-
-
-
-
-
-
-
-
1
-
19
-
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-
-
-
1
-
2
-
2
1
-
-
-
1
-
-
-
-
-
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-
1
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1
-
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-
-
-
1
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
677441
Loupatty
Mutations in the gene encoding ...
Homo sapiens
Am. J. Hum. Genet.
80
195-199
2007
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
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-
-
-
-
-
-
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1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
665823
Iida
Further application of a two-s ...
Homo sapiens
J. Chromatogr. B
823
209-212
2005
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
1
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1
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1
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1
-
1
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1
-
1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651589
Wong
Divergent function in the crot ...
Pseudomonas aeruginosa
J. Am. Chem. Soc.
125
12076-12077
2003
-
-
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-
4
-
-
-
-
-
-
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-
1
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1
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-
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-
1
-
3
-
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3
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4
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-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
650865
Ishigure
Human liver disease decreases ...
Homo sapiens
Clin. Chim. Acta
312
115-121
2001
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
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-
-
4
1
-
2
-
2
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1
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4
1
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2
-
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-
-
-
-
-
-
-
-
-
-
-
-
653127
Shimomura
3-Hydroxyisobutyryl-CoA hydrol ...
Homo sapiens, Rattus norvegicus
Methods Enzymol.
324
229-240
2000
-
-
1
-
-
-
8
2
1
-
3
2
-
2
-
-
2
-
-
6
3
-
9
2
-
1
-
-
-
2
1
-
-
8
-
-
-
-
1
-
-
-
-
-
8
8
2
1
-
3
2
-
-
-
2
-
6
3
-
9
2
1
-
-
-
2
1
-
-
-
-
-
-
-
-
94557
Hawes
Primary structure and tissue-s ...
Rattus norvegicus, Homo sapiens
J. Biol. Chem.
271
26430-26434
1996
-
-
1
-
-
-
-
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2
3
-
12
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7
2
2
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-
-
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-
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1
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-
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2
3
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-
-
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-
7
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94556
Ooiwa
Regulation of valine catabolis ...
Canis lupus familiaris
Biochim. Biophys. Acta
1243
216-220
1995
-
-
-
-
-
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-
-
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6
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8
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1
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8
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1
-
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-
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-
-
-
-
94558
Shimomura
Purification and partial chara ...
Rattus norvegicus
J. Biol. Chem.
269
14248-14253
1994
-
-
-
-
-
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8
2
1
-
1
1
-
4
-
-
1
-
-
5
1
-
4
1
-
-
-
-
2
2
2
-
-
-
-
-
-
-
-
-
-
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-
-
8
-
2
1
-
1
1
-
-
-
1
-
5
1
-
4
1
-
-
-
2
2
2
-
-
-
-
-
-
-
-
94555
Nurmikko
-
Product induction of 3-hydroxy ...
Pseudomonas fluorescens, Pseudomonas fluorescens UK-1
Suom. Kemistil. B
45
193-196
1972
-
-
-
-
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-
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2
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4
-
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4
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1
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2
-
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4
-
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