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Literature summary for 3.1.2.4 extracted from

  • Shimomura, Y.; Murakami, T.; Nakai, N.; Huang, B.; Hawes, J.W.; Harris, R.A.
    3-Hydroxyisobutyryl-CoA hydrolase (2000), Methods Enzymol., 324, 229-240.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli BL21(DE3) as His-tagged enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
acetoacetyl-CoA competitive inhibition Homo sapiens
acetyl-CoA competitive inhibition Homo sapiens
DL-3-hydroxybutyryl-CoA competitive inhibition Homo sapiens
DL-methylmalonyl-CoA competitive inhibition Homo sapiens
isobutyryl-CoA competitive inhibition Homo sapiens
malonyl-CoA competitive inhibition Homo sapiens
n-valeryl-CoA competitive inhibition Homo sapiens
propionyl-CoA competitive inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
(S)-3-hydroxy-2-methylpropanoyl-CoA recombinant enzyme, pH 8.0, 30°C Homo sapiens
0.025
-
3-hydroxypropionyl-CoA recombinant enzyme, pH 8.0, 30°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
gel filtration Rattus norvegicus
38000
-
1 * 39398, amino acid sequence calculation, 1 * 38000, recombinant enzyme, SDS-PAGE Homo sapiens
39398
-
1 * 39398, amino acid sequence calculation, 1 * 38000, recombinant enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxy-2-methylpropanoyl-CoA + H2O Homo sapiens
-
CoA + 3-hydroxy-2-methylpropanoate
-
?
3-hydroxy-2-methylpropanoyl-CoA + H2O Rattus norvegicus substrate is an intermediate in the pathway of valine catabolism, which is unique in that it produces a radily diffusible monocarboxylic acid 3-hydroxy-2-methylpropanoate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from rat liver, 7200fold, to homogeneity Rattus norvegicus
recombinant enzyme from Escherichia coli, removal of His-tag with thrombin Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-
heart
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Homo sapiens
-
liver
-
Rattus norvegicus
-
muscle
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
244
-
purified recombinant enzyme, substrate 3-hydroxypropionyl-CoA Homo sapiens
427
-
purified enzyme Rattus norvegicus
430
-
purified recombinant enzyme, substrate (S)-3-hydroxy-2-methylpropanoyl-CoA Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxy-2-methylpropanoyl-CoA + H2O
-
Homo sapiens CoA + (S)-3-hydroxy-2-methylpropanoate
-
?
3-hydroxy-2-methylbutyryl-CoA + H2O low activity Homo sapiens CoA + 3-hydroxy-2-methylbutyrate
-
?
3-hydroxy-2-methylpropanoyl-CoA + H2O
-
Homo sapiens CoA + 3-hydroxy-2-methylpropanoate
-
?
3-hydroxy-2-methylpropanoyl-CoA + H2O
-
Rattus norvegicus CoA + 3-hydroxy-2-methylpropanoate
-
?
3-hydroxy-2-methylpropanoyl-CoA + H2O substrate is an intermediate in the pathway of valine catabolism, which is unique in that it produces a radily diffusible monocarboxylic acid Rattus norvegicus 3-hydroxy-2-methylpropanoate + CoA
-
?
3-hydroxypropanoyl-CoA + H2O
-
Homo sapiens CoA + 3-hydroxypropanoate
-
?
DL-3-hydroxybutyryl-CoA + H2O low activity Homo sapiens CoA + DL-3-hydroxybutyrate
-
?
L-3-hydroxybutyryl-CoA + H2O low activity Homo sapiens CoA + L-3-hydroxybutyrate
-
?
additional information substrate specificity, overview, acetoacetyl-CoA, DL-methylmalonyl-CoA, isobutyryl-CoA, malonyl-CoA, acetyl-CoA, propionyl-CoA, and n-valeryl-CoA are poor substrates Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 36000, SDS-PAGE Rattus norvegicus
monomer 1 * 39398, amino acid sequence calculation, 1 * 38000, recombinant enzyme, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Homo sapiens
8
-
assay at Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5.1 10.3 at least Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.12
-
acetoacetyl-CoA recombinant enzyme, pH 8.0, 30°C Homo sapiens
0.14
-
DL-3-hydroxybutyryl-CoA recombinant enzyme, pH 8.0, 30°C Homo sapiens
0.72
-
n-valeryl-CoA recombinant enzyme, pH 8.0, 30°C Homo sapiens
0.83
-
propionyl-CoA recombinant enzyme, pH 8.0, 30°C Homo sapiens
1.19
-
isobutyryl-CoA recombinant enzyme, pH 8.0, 30°C Homo sapiens
1.6
-
malonyl-CoA recombinant enzyme, pH 8.0, 30°C Homo sapiens
2.26
-
DL-methylmalonyl-CoA recombinant enzyme, pH 8.0, 30°C Homo sapiens
2.61
-
acetyl-CoA recombinant enzyme, pH 8.0, 30°C Homo sapiens